_I_T11_dCas9SynProm

Short Name: T11_dCas9SynProm Title: Development of synthetic promoters inducible by dCasEV2.1 system Description: The aim of this work is to design a range of synthetic promoters with negligible basal expression that are activated by using the "dead" Cas9 activation system developed in our lab (dCasEV2.1, Selma et al., 2019). Such tool will then be used for creating synthetic regulatory cascades where the expression of the genes for the pheromone biosynthesis can be controlled by a single dCas9 gene at custom expression levels. By adding as much variability as possible to each synthetic promoter, issues arising from the use of similar promoters very close to each other's will be avoided. We also aim to have synthetic promoters with different strength to further modulate the biosynthetic pathways. Phenodata: ./phenodata_Luciferase_20211222.txt Principal investigator: Diego Orzaez License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes

SEEK ID: https://fairdomhub.org/investigations/506

Projects: SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Contr...

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Created: 10th Jan 2022 at 10:14

Last updated: 7th Nov 2024 at 10:01

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