_A_20191209-LUM

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Short Name: 20191209-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay third trial Description: In order to imitate better the native DFR promoter, all upstream promoter sequences were re-designed as A2 pieces and A1 pieces were ordered which consisted on just random sequences witout any promoter signals. Assembling these pieces created synthetic promoters of the same length than the original SlDFR. Due to some assembly issues, all new constructs were made using 35s terminator. pISA Assay creation date: 2019-09-12 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to protocols: ../protocols/LUCIFERASE Additional Notes: Phenodata: ../../phenodata_Luciferase_20211222.txt Featuredata: Plate ID: 03 DNA introduction system: agrobacteria mediated transient transformation Date of transformation/transfection: 2019-09-07 Treatment: none Date of treatment: 2019-09-12 Date of sampling: 2019-09-12 Date of luminescence assay start: 2021-11-20 Time of luminescence assay start: 12:00 Luminometer: GloMax Multidetection system (Promega) Protocol (luminometer setup): Luminiscence 3x read Time [min] between two measurements (luminometer setup): 2 Integration time [ms] (luminometer setup): 10000 Temperature (luminometer) at start of the experiment [degrees Celsius]: 24 Temperature (luminometer) at the end of the experiment [degrees Celsius]: 24 Time between start of sampling and start of experiment [min]: 60 Silencing supressor added: p19 Notes: Data:

SEEK ID: https://fairdomhub.org/assays/1621

Class: Experimental assay

Projects: SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Contr...

Investigation: _I_T11_dCas9SynProm

Study: _S_P1_SynProm_Nbenthamiana

Assay position:

Assay type: Experimental Assay Type

Technology type: Technology Type

Organisms: No organisms

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