Assays

Short Name: 20200818-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay of new minimal promoters Description: New minimal promoters were designed and tested to increase the battery of pieces available for creating synthetic promoters pISA Assay creation date: 2020-08-18 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to protocols: ../protocols/LUCIFERASE ...

Short Name: 20201112-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay re-testing US6.X Description: The US6.X series was tested again using separated plasmids for the gRNAs and the dCas9EV system (so far it was used a plasmid containig the system and the guide together) pISA Assay creation date: 2020-11-12 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 ...

Short Name: 20211221-LUM Assay Class: WET Assay Type: LUM Title: dCasEV synthetic promoters with NanoLuc reporter system Description: Test for dCasEV synthetic promoters using EI reporter system based on Nanoluciferase as reporter and luciferase as reference signal. pISA Assay creation date: 2021-12-22 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to ...

Short Name: 20190208-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay first trial Description: Promoter series containing random sequences and the recognition sequence for guideRNA1 DFR (P1.X series) fused to the developed core promoter minDFR and to luciferase for measuring the expression in the presence or absence of such guideRNA1 DFR. A promoter containing the target sequence in a different position (P1.7.1) and a promoter with the target sequence repeated twice (P2.1) were also ...

Short Name: 20190717-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay second trial Description: New 1.X and P2.X promoter sequences, and also a P3.1 promoter containing three times the target sequence for guideRNA1 DFR pISA Assay creation date: 2019-07-17 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: https://docs.google.com/spreadsheets/d/1KqJ2s7WZOro2pTCjGuUDpnSRfum26omN1TRFDl6-gAI/edit#gid=1915967703 Link to protocols: ../protocols/LUCIFERASE ...

Short Name: 20191209-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay third trial Description: In order to imitate better the native DFR promoter, all upstream promoter sequences were re-designed as A2 pieces and A1 pieces were ordered which consisted on just random sequences witout any promoter signals. Assembling these pieces created synthetic promoters of the same length than the original SlDFR. Due to some assembly issues, all new constructs were made using 35s terminator. pISA ...

Short Name: 20200219-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay of different A1 random sequences Description: With the aim of making three promoters as different as possible for the moth pheromone enzymes, three A1 pieces containing random sequences withous any promoter signal were assembled and tested using luciferase assay. pISA Assay creation date: 2020-02-19 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio DM: ...

Short Name: 20200302-LUM Assay Class: WET Assay Type: LUM Title: Luciferase Assay US1.7.X and US3.X pieces Description: Analysis of new US pieces with three recognition sites for gRNA1 DFR (US3.X) and US pieces with the recognition site in different positions (US1.7.1-US1.7.4), as well as another US part with the recognition site of another gRNA (US1.7.5, for gRNA4 from NOS promoter). pISA Assay creation date: 2020-02-03 pISA Assay creator: ElenaMG Lab manager: DO Operator: ElenaMG Link to SynBio ...

Files connected to investigation: _I_T11_dCas9SynProm

Files connected to study: _S_P1_SynProm_Nbenthamiana