4 seeds of stable NanoLUC T3 homozygous lines for LHY, TOC1 and ELF3 were seeded in 96-well flat white plate that contained 150 µl of ROBUST media and stratified for 2 days at 4ºC. Then a 2 hours pulse of white light given and transferred to 22 hours darkness at 21 ºC. Then transferred 12L:12D photoperiod for 10 days at which 50 µl of 1:50 Furimazine:0.05% Triton X-100 added to each well for tracking NanoLUC bioluminescence. Measurements using a Tristar plate reader were performed automatically every hour for three days in 12:L:12D and then light conditions were set to constant
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Created: 11th Jun 2023 at 11:58
Last updated: 19th Dec 2024 at 11:28
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Version 1 (earliest) Created 11th Jun 2023 at 11:58 by Uriel Urquiza Garcia
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