Clock_protein_time_series_12L_12D
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4 seeds of stable NanoLUC T3 homozygous lines for LHY, PRR7, TOC1, and ELF3 were seeded in 96-well flat white plate that contained 150 µl of ROBUST media and stratified for 2 days at 4ºC. Then plates were incubated in a 2 hours pulse of white light given and transferred to 22 hours darkness at 21 ºC. Then transferred 12L:12D photoperiod for 10 days. On day 10, 50 µl of 1:50 Furimazine:0.05% Triton X-100 added to each well for tracking NanoLUC bioluminescence. A) Measurements using a Tristar plate reader were performed automatically every hour under 12L:12D ~100 µmol/m^2s of cool fluorescent white light at 21ºC

SEEK ID: https://fairdomhub.org/data_files/6536?version=1

Filename: 120419t.xlsx