_ASSAY_METADATA.TXT

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National Institute of Biology, Department of Biotechnology and Systems Biology projects

SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.

Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036

Short Name: T21_SXPsysbio Title: Use a systems biology approach to identify regulatory bottlenecks in SxPv1 Description: Samples from SXPv1.0 plants as well as sister nulls (progeny from the original transgenic event in which the transgene has segregated) and wild type will be grown and leaf samples taken for RNA extraction and profiling of primary metabolites and volatiles (target pheromones as well as potential derivatives) (P1, P5). Phenotypic and GC-MS data will be obtained and analysed from ...

Short Name: Gibberelin_treatment Title: GA3 treatment of SxPs Description: SxP lines were treated with 3 different GA3 concentrations (1, 10 and 100 micromolar) and control solution. WT Nb plants were used for control. From v1.0 T3, low producing line 5_2_5 and high producing line 5_1_7 were used and from v1.2 T2 lines 4_1 and 4_3. Treatment was done for 5 consecutive weeks, once per week with spraying the leaves. Raw Data: Principal investigator: Ĺ pela Baebler License: Creative Commons Attribution ...

Assay: AP4v10v12-phenotyping Short Name: P4v10v12-phenotyping Assay Class: WET Assay Type: phenotyping Title: Phenotyping of GA3 effect on SxP lines in comparison to wt Description: GA3 treated plants were followed for 35 days after transplanting in soil. Plant height was measured at 17, 21, 24, 28 and 35 days post planting in soil. At day 35 in soil, plants were collected, weighed and photographed for image analysis of leaf areas. The leaves and stems were dried and dry weigh measured. pISA Assay ...