_ASSAY_METADATA.TXT

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National Institute of Biology, Department of Biotechnology and Systems Biology projects

SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.

Short Name: T11_dCas9SynProm Title: Development of synthetic promoters inducible by dCasEV2.1 system Description: The aim of this work is to design a range of synthetic promoters with negligible basal expression that are activated by using the "dead" Cas9 activation system developed in our lab (dCasEV2.1, Selma et al., 2019). Such tool will then be used for creating synthetic regulatory cascades where the expression of the genes for the pheromone biosynthesis can be controlled by a single dCas9 ...

Short Name: P1_SynProm_Nbenthamiana Title: Transient expression in Nicotiana benthamiana of the synthetic promoters developed Description: Different A1 and A2 Different A1 and A2 promoter pieces were designed containing as much random sequence as possible, and the target sequence for the guideRNA 1 of SlDFR promoter. In the same way, some minimal promoters containing only the TATA-Box signal and the 5'UTR region were designed as "core promoter" pieces. Different synthetic promoters were then ...

Short Name: 20191209-LUM Assay Class: WET Assay Type: LUM Title: Luciferase assay third trial Description: In order to imitate better the native DFR promoter, all upstream promoter sequences were re-designed as A2 pieces and A1 pieces were ordered which consisted on just random sequences witout any promoter signals. Assembling these pieces created synthetic promoters of the same length than the original SlDFR. Due to some assembly issues, all new constructs were made using 35s terminator. pISA ...