RNA processing in Bacillus subtilis: Identification of targets of the essential RNase Y
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RNA processing and degradation is initiated by endonucleolytic cleavage of the target RNAs. In many bacteria, this activity is performed by RNase E which is not present in Bacillus subtilis and other Gram-positive bacteria. Recently, the essential endoribonuclease RNase Y has been discovered in B. subtilis. This RNase is involved in the degradation of bulk mRNA suggesting a major role in mRNA metabolism. However, only a few targets of RNase Y have been identified so far. In order to assess the global impact of RNase Y, we compared the transcriptomes of strains expressing RNase Y or depleted for RNase Y. Our results indicate that processing by RNase Y results in accumulation of about 80 mRNAs. Some of these targets were substantially stabilized by RNase Y depletion, resulting in half-lives in the range of an hour. Moreover, about 40 mRNAs were less abundant when RNase Y was depleted among them the mRNAs of the operons required for biofilm formation. Interestingly, overexpression of RNase Y was sufficient to induce biofilm formation. The results emphasize the importance of RNase Y for B. subtilis and are in support of the idea that RNase Y is the functional equivalent of RNase E.

This file contains processed data (gene expression ratios), original data file "Trancriptome data - RNA processing in Bacillus subtilis: identification of targetsof the essential RNase Y" (https://seek.sysmo-db.org/data_files/1051) uploaded to Gene Expression Omnibus database.

SEEK ID: https://fairdomhub.org/data_files/1055?version=1

Filename: Lehnik et al 2011 Table S1.xls  Download

Format: Spreadsheet

Size: 109 KB

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Created: 19th Dec 2012 at 10:29

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