Collection of clock models that rescale transcript variables to account for absolute units. The relationship between models is summarised in the attached 'model evolution' document and in more detail in the linked publications. Each model is presented three times, - without a light:dark cycle, - with an ISSF (Adams et al. JBR 2012) that is set up for LD 12h:12h cycles, and - with an ISSF that runs 10 LD cycles followed by an SBML Event that switches to constant light, matching published simulations. ...

Transcriptome analysis suggests a compensatory role of the cofactors coenzyme A and NAD+ in medium-chain acyl-CoA dehydrogenase knockout mice. During fasting, mitochondrial fatty-acid β-oxidation (mFAO) is essential for the generation of glucose by the liver. Children with a loss-of-function deficiency in the mFAO enzyme medium-chain acyl-Coenzyme A dehydrogenase (MCAD) are at serious risk of life-threatening low blood glucose levels during fasting in combination with intercurrent disease. However, ...

The procedure describes the preparation of fluorescent DNA probes from human mRNA or total RNA.