Glucose starvation specific responses – Quantification of thermodynamic and kinetic parameters of the CcpA regulation network
- Order Assays
Created: 7th Mar 2011 at 14:27
Last updated: 22nd Nov 2011 at 20:12
Expertise: carbon catabolite regulation in Gram positive bacteria, interaction analyses of transcriptional regulators, analysis and preparation of diagnostic markers for Helicobacter pylori infections Yersiniosis and collagenoses
I studied chemistry in Erlangen and started research on carbon catabolite regulation in Bacillus megaterium in my diploma thesis. During my PhD thesis I was concerned with quantitative analyses of protein-protein and protein-DNA interactions of CcpA, in vivo- and in vitro-characterization of point mutants of CcpA and coeffectors and I contributed to the structural analysis of different CcpA complexes. During a postdoctoral appointment I focused on interaction analyses of PrfA, a regulator of ...
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Web page: http://sysmo.net/
BaCell-SysMO 2 Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying ...
The aim of this project is to develop a detailed kinetic model of the CcpA-dependent regulatory network, the key regulon of flux regulation in B. subtilis. Thereby involved are more than 300 genes e.g. catabolism, overflow metabolism, the TCA cycle and amino acid anabolism which are regulated via carbon catabolite regulation (CCR)
Submitter: Gerald Seidel
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For analyzing the binding of CcpA-HPrSer46P-complexes to various cre-elements, Surface Plasmon Resonance was used. All operations were carried out on a Biacore X instrument (Biacore, Uppsala, Sweden). Biotinylated cre DNA was coupled on a Neutravidin coated chip in flowcell two, a biotinylated reference DNA in flowcell one. For visualizing only the interactions of the CcpA-HPrSer46P-complex with the cre elements, CcpA was saturated with 50µM HPrSer46P. Titrations were carried out with 5-100nM ...
Submitter: Maike Bartholomae
Assay type: Protein-protein Interaction
Technology type: Surface Plasmon Resonance
Investigation: A dynamic model of the CcpA regulation network
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
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