Assays
What is an Assay?Filters
Query
Created At
Updated At
Project
BaCell-SysMO1
Submitter
Maike Bartholomae1
Assay class
Experimental assay1
Assay type
Protein-protein Interaction1
Technology type
Surface Plasmon Resonance1
1
Assay visible to you, out of a total of 5
For analyzing the binding of CcpA-HPrSer46P-complexes to various cre-elements, Surface Plasmon Resonance was used. All operations were carried out on a Biacore X instrument (Biacore, Uppsala, Sweden). Biotinylated cre DNA was coupled on a Neutravidin coated chip in flowcell two, a biotinylated reference DNA in flowcell one. For visualizing only the interactions of the CcpA-HPrSer46P-complex with the cre elements, CcpA was saturated with 50µM HPrSer46P. Titrations were carried out with 5-100nM ...
Submitter: Maike Bartholomae
Assay type: Protein-protein Interaction
Technology type: Surface Plasmon Resonance
Investigation: A dynamic model of the CcpA regulation network
