Preparation of DNA-free RNA prior to RT-PCR Version 1
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
SEEK ID: https://fairdomhub.org/sops/26?version=1
Filename:
Preparation of DNA-free RNA.pdf
Format: PDF document
Size: 8.27 KB
Export PNG
Creators and Submitter
Views: 2455 Downloads: 72
Created: 26th Oct 2009 at 14:48
TagsThis item has not yet been tagged.
AttributionsNone
Version History
Version 1 (earliest) Created 26th Oct 2009 at 14:48 by Lisbeth Lyngberg
No revision comments
Related items
Projects: KOSMOBAC
Institutions: University of Aberdeen
I am a research technician at the Institute of Medical Science in Aberdeen, working for Prof. Ian Booth. The topic of our workpackage deals with K+ homostasis in Escherichia coli. I am working with the protein KefF, a regulatory subunit of the potassium channel KefC.
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Ion and solute homeostasis in enteric bacteria: an integrated view generated from the interface of modelling and biological experimentation
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=56
