D.PCR-250520KAM-1
2D_PCR_day10_analysis.xlsx
https://zenodo.org/records/15475937/files/2D_PCR_day10_analysis.xlsx?download=1&preview=1
9688cfcd122c9104783cc8030b2f1223
Elena Cambria
Real-time RT-PCR
RNA-250520KAM-1; RNA-250520KAM-2; RNA-250520KAM-3; RNA-250520KAM-4; RNA-250520KAM-5; RNA-250520KAM-6
https://fairdomhub.org/sops/871
RNeasy Mini Kit (Qiagen)
Applied Biosystems software
SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher)
Integrated DNA Technologies: SEE PROTOCOL
Massachusetts General Hospital Primer Bank: SEE PROTOCOL
Applied Biosystems 7300 Fast Real-Time PCR System
h36b4
2-delta delta Ct method
ACTA2, COL1A1, FN1
Creators and SubmitterViews: 6
Created: 5th Jan 2026 at 19:17
Last updated: 5th Jan 2026 at 19:50
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Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab
Institutions: Massachusetts Institute of Technology
Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab
Web page: https://nextseek.mit.edu/
Understanding the spectrum of complex metastatic processes is important to the development of a comprehensive and cohesive understanding of cancer metastasis. The Metastasis Research Network (MetNet) supports collaborative, multidisciplinary projects that focus on several themes of the metastatic process and use integrative systems-level approaches.
MetNet is advancing the understanding of metastasis as a non-linear, dynamic, and emergent process, as well as revealing mechanistic insights of early ...
Programme: NExtSEEK
Public web page: Not specified
Submitter: Charles Demurjian
Studies: Chemo-mechanical diffusion waves explain collective dynamics of immune c..., Chromatin conformation, gene transcription, and nucleosome remodeling as..., Confined cell migration along extracellular matrix space in vivo, Impact of fibrinogen, fibrin thrombi and thrombin on cancer cell extrava..., Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized ..., Patient-specific vascularized tumor model: Blocking monocyte recruitment..., Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Bio..., Polyploidy of MDA-MB-231 cells drives increased extravasation with enhan..., Remodeling of self-assembled microvascular networks under long term flow, Utilizing convolutional neural networks for discriminating cancer and st...
Assays: All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, Cancer Cell Extravasation Analysis - Data Linked, Cancer Cell Extravasation Analysis - Data Linked, Cell Culture - Metadata, Cell Culture - Metadata, Cell Culture Imaging - Data Linked, Cell Culture Imaging Analysis - Data Linked, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Extraction - Metadata, Cell Imaging - Data Linked, Chromatin Sequencing Analysis - Data Linked, Clot Modeling Analysis - Data Linked, Convolutional Neural Network - Data Linked, Cytokine Luminex - Data Linked, Dendritic Cell Preparation - Metadata, Device Creation - Metadata, Device Creation - Metadata, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Metadata, ELISA - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Fluorescence-Activated Cell Sorting - Data Linked, Gene Expression Analysis - Data Linked, Gene Expression Analysis - Data Linked, Imaging - Data Linked, Imaging Analysis - Data Attached, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Intravital Microscopy, Library Creation - Metadata, Library Prep - Metadata, Library Prep - Metadata, Live Cell Imaging - Data Linked, Luminex - Data Linked, MVN Imaging Analysis - Data Linked, Mass Spectrometry Proteomics - Data Linked, Microfluidic Device Creation - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Imaging - Data Linked, Microfluidic Network Formation - Metadata, Microfluidic Network Formation - Metadata, Microfluidic Network Imaging - Data Linked, Microvascular Network Formation - Metadata, Migration Assay Analysis - Data Linked, Modeling - Data Linked, Modeling - Data Linked, Permeability Analysis - Data Linked, Proteomics Analysis - Data Linked, RNA Extraction - Metadata, RNA Extraction - Metadata, RNA Extraction - Metadata, Real Time RT-PCR - Data Linked, Real-time RT-PCR - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Tumoroid Formation - Metadata
Elena Cambria, Adriana Blazeski, Eunkyung Clare Ko, Tran Thai, Shania Dantes, David A. Barbie, Sarah E. Shelton, Roger D. Kamm
https://www.biorxiv.org/content/10.1101/2025.01.10.632378v1
Abstract: Lung fibrosis, characterized by chronic and progressive scarring, has no cure. Hallmarks are the accumulation of myofibroblasts and extracellular matrix, as well as vascular remodeling. The crosstalk between myofibroblasts and vasculature is poorly understood, with ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
