D.IMG-250515FRI-1
samples-publish_PF-IVM_D.IMG.xlsx
Raw Intravital Microscopy Images can be aquired by reaching out to the corresponding author
Peter Friedl
Corresponding Author: caterina.laporta@unimi.it
Intravital multiphoton microscopy (MPM)
https://fairdomhub.org/sops/830
MUS-250515FRI-1
LaVision BioTec multiphoton microscope
20x magnification, NA 0.95 (Olympus)
7 days post-surgery
6 Minuetes
Approximately 4 hours
H2B-EGFP, DsRed2
910 nm for eGFP, 1090 nm for DsRed2 and SHG
Nuclear Marker: H2B-EGFP (green), Cytoplasmic Marker: DsRed2 (red)
Creators and SubmitterViews: 35
Created: 15th May 2025 at 18:58
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Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Collagen
Institutions: Massachusetts Institute of Technology
Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Collagen
Web page: https://nextseek.mit.edu/
Submitter: Charles Demurjian
Studies: Chromatin conformation, gene transcription, and nucleosome remodeling as..., Confined cell migration along extracellular matrix space in vivo, Impact of fibrinogen, fibrin thrombi and thrombin on cancer cell extrava..., Patient-specific vascularized tumor model: Blocking monocyte recruitment..., Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Bio..., Polyploidy of MDA-MB-231 cells drives increased extravasation with enhan..., Utilizing convolutional neural networks for discriminating cancer and st...
Assays: All Metadata, All Metadata, All Metadata, All Metadata, Cancer Cell Extravasation Analysis - Data Linked, Cancer Cell Extravasation Analysis - Data Linked, Cell Culture - Metadata, Cell Culture - Metadata, Cell Culture Imaging - Data Linked, Cell Culture Imaging Analysis - Data Linked, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Imaging - Data Linked, Chromatin Sequencing Analysis - Data Linked, Clot Modeling Analysis - Data Linked, Convolutional Neural Network - Data Linked, Device Creation - Metadata, Device Creation - Metadata, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Metadata, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Fluorescence-Activated Cell Sorting - Data Linked, Gene Expression Analysis - Data Linked, Imaging Analysis - Data Attached, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Intravital Microscopy, Library Creation - Metadata, Library Prep - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Imaging - Data Linked, Microvascular Network Formation - Metadata, Migration Assay Analysis - Data Linked, Modeling - Data Linked, Permeability Analysis - Data Linked, RNA Extraction - Metadata, Real-time RT-PCR - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Tumoroid Formation - Metadata
Oleksandr Chepizhkoa, Josep-Maria Armengol-Colladob, Stephanie Alexander, Esther Wagenae, Bettina Weigelin, Luca Giomi, Peter Friedl, Stefano Zapperi,Caterina A. M. La Porta
DOI: https://doi.org/10.1073/pnas.2414009121
Abstract: Cancer metastasis involves the collective migration of cancer cells in confined space, a problem that has been widely investigated in vitro. Here, we quantify the flow patterns of cancer cell as they invade tissue space and deform ...
Submitter: Charles Demurjian
Investigation: MetNet
Assays: All Metadata, Imaging Analysis - Data Linked, Intravital Microscopy
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
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