D.PCR-240715KAM-1
MDA 231 EGFP Positive
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM8392373
Satomi Hirose
Real-time RT-PCR
RNA-240709KAM-1
Gene Expression Omnibus
GSE272066
https://fairdomhub.org/sops/741
RNeasy Plus Mini Kit (74134; Qiagen)
Verso cDNA Synthesis Kit (AB1453A; Thermo Fisher Scientific)
Custom DNA oligonucleotides (Integrated DNA Technologies)
Table S1
Quantstudio 3 Real-Time PCR System (Thermo Fisher Scientific)
Peptidylprolyl isomerase A (PPIA)
2-delta delta Ct method
Creators and SubmitterViews: 11
Created: 15th Jul 2024 at 20:00
Last updated: 15th Jan 2025 at 19:10
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Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC)
Institutions: Massachusetts Institute of Technology
Programme: Metastasis Network
Public web page: Not specified
Submitter: Charles Demurjian
Studies: Impact of fibrinogen, fibrin thrombi and thrombin on cancer cell extrava..., Patient-specific vascularized tumor model: Blocking monocyte recruitment..., Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Bio..., Polyploidy of MDA-MB-231 cells drives increased extravasation with enhan..., Utilizing convolutional neural networks for discriminating cancer and st...
Assays: All Metadata, All Metadata, Cancer Cell Extravasation Analysis - Data Linked, Cancer Cell Extravasation Analysis - Data Linked, Cell Culture - Metadata, Cell Culture Imaging - Data Linked, Cell Culture Imaging Analysis - Data Linked, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Culture and Tumor Spheroid Creation - Metadata, Clot Modeling Analysis - Data Linked, Convolutional Neural Network - Data Linked, Device Creation - Metadata, Device Creation - Metadata, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Metadata, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Fluorescence-Activated Cell Sorting - Data Linked, Gene Expression Analysis - Data Linked, Imaging Analysis - Data Attached, Library Prep - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Imaging - Data Linked, Microvascular Network Formation - Metadata, Migration Assay Analysis - Data Linked, Permeability Analysis - Data Linked, RNA Extraction - Metadata, Real-time RT-PCR - Data Linked, Short Read Sequencing - Data Linked, Tumoroid Formation - Metadata
Satomi Hirose, Tatusya Osaki, Roger D. Kamm
Link to Publication: https://doi.org/10.1101/2024.06.28.601261
Abstract: Metastasis, the leading cause of cancer-related deaths, involves a complex cascade of events, including extravasation. Despite extensive research into metastasis, the mechanisms underlying extravasation remain unclear. Molecular targeted therapies have advanced cancer treatment, yet their efficacy is limited, prompting exploration into novel ...
Submitter: Charles Demurjian
Investigation: MetNet
Assays: All Metadata, Cancer Cell Extravasation Analysis - Data Linked, Cell Culture - Metadata, Cell Culture Imaging - Data Linked, Cell Culture Imaging Analysis - Data Linked, Fluorescence-Activated Cell Sorting - Data Linked, Gene Expression Analysis - Data Linked, Library Prep - Metadata, Microfluidic Device Imaging - Data Linked, Microvascular Network Formation - Metadata, RNA Extraction - Metadata, Real-time RT-PCR - Data Linked, Short Read Sequencing - Data Linked
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
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