Molecular sieving properties of the cytoplasm of Escherichia coli and consequences of osmotic stress

Abstract:

We determined the diffusion coefficients (D) of (macro)molecules of different sizes (from ∼0.5 to 600 kDa) in the cytoplasm of live Escherichia coli cells under normal osmotic conditions and osmotic upshift. D values decreased with increasing molecular weight of the molecules. Upon osmotic upshift, the decrease in D of NBD-glucose was much smaller than that of macromolecules. Barriers for diffusion were found in osmotically challenged cells only for GFP and larger proteins. These barriers are likely formed by the nucleoid and crowding of the cytoplasm. The cytoplasm of E. coli appears as a meshwork allowing the free passage of small molecules while restricting the diffusion of bigger ones.

SEEK ID: https://fairdomhub.org/publications/119

DOI: 10.1111/j.1365-2958.2010.07201.x

Projects: KOSMOBAC

Publication type: Not specified

Citation:

Date Published: 1st Jul 2010

Registered Mode: Not specified

Authors: , Geert Van Den Bogaart, Liesbeth Veenhoff, Victor Krasnikov,

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Citation
Mika, J. T., Van Den Bogaart, G., Veenhoff, L., Krasnikov, V., & Poolman, B. (2010). Molecular sieving properties of the cytoplasm of Escherichia coli and consequences of osmotic stress. In Molecular Microbiology (Vol. 77, Issue 1, pp. 200–207). Wiley. https://doi.org/10.1111/j.1365-2958.2010.07201.x
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Created: 28th May 2011 at 14:46

Last updated: 8th Dec 2022 at 17:26

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