Peter Dürre

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About Peter Dürre:

Prof. Dr. Peter Dürre Head, Department of Microbiology and Biotechnology, University of Ulm 89069 Ulm, Germany

SEEK ID: https://fairdomhub.org/people/23

Location: Germany

ORCID: Not specified

Joined: 21st Apr 2009

Expertise: Microbiology Biochemistry Molecular biology Genetics Enzymology Synthetic biology Regulatory metabolic networks

Tools: DNA technology RNA technology Protein analysis Fermentation Mutagenesis

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Era CoBioTech

ERA CoBioTech is an ERA-Net Cofund Action under H2020, which aims to strengthen the European Research Area (ERA) in the field of Biotechnology through enhanced cooperation and coordination of different national and regional research programs, promoting systems biology and synthetic biology as technology driversto speed up research and innovation in industrial biotechnology.

› By maximizing synergies between current mechanisms of biotechnology research funding in Europe; › By fostering the exchange ...

Projects: Sustainable co-production, BESTER, Rhodolive, HOTSOLUTE, TRALAMINOL, C1Pro, Cell4Chem

Web page: https://www.cobiotech.eu/

SysMO

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep

Web page: http://sysmo.net/

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BESTER

Bioprocesses for the optimized, integrated production of butyl esters from sustainable resources (BESTER)

Industrial Biotechnology is a key enabling technology to produce a plethora of different bio-based products from sustainable resources and a driver for developing the bio-based economy in Europe. Systems biology and Synthetic biology are recent additions to the biotechnology toolbox that in interplay with bioprocess and chemical process technology can help developing competitive industrial ...

Programme: Era CoBioTech

Public web page: https://www.cobiotech.eu/call-information

Organisms: Clostridia Sp.

COSMIC

Systems Biology of Clostridium acetobutylicum - a possible answer to dwindling crude oil reserves

Programme: SysMO

Public web page: http://www.sysmo.net/index.php?index=54

Organisms: Clostridium acetobutylicum

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University of Ulm

Country: Germany

City: Ulm

Web page: http://www.uni-ulm.de/

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Energy Conservation Associated with Ethanol Formation from H2 and CO2 in Clostridium autoethanogenum Involving Electron Bifurcation.

BESTER

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UNLABELLED: Most acetogens can reduce CO2 with H2 to acetic acid via the Wood-Ljungdahl pathway, in which the ATP required for formate activation is regenerated in the acetate kinase reaction. However, … a few acetogens, such as Clostridium autoethanogenum, Clostridium ljungdahlii, and Clostridium ragsdalei, also form large amounts of ethanol from CO2 and H2. How these anaerobes with a growth pH optimum near 5 conserve energy has remained elusive. We investigated this question by determining the specific activities and cofactor specificities of all relevant oxidoreductases in cell extracts of H2/CO2-grown C. autoethanogenum. The activity studies were backed up by transcriptional and mutational analyses. Most notably, despite the presence of six hydrogenase systems of various types encoded in the genome, the cells appear to contain only one active hydrogenase. The active [FeFe]-hydrogenase is electron bifurcating, with ferredoxin and NADP as the two electron acceptors. Consistently, most of the other active oxidoreductases rely on either reduced ferredoxin and/or NADPH as the electron donor. An exception is ethanol dehydrogenase, which was found to be NAD specific. Methylenetetrahydrofolate reductase activity could only be demonstrated with artificial electron donors. Key to the understanding of this energy metabolism is the presence of membrane-associated reduced ferredoxin:NAD(+) oxidoreductase (Rnf), of electron-bifurcating and ferredoxin-dependent transhydrogenase (Nfn), and of acetaldehyde:ferredoxin oxidoreductase, which is present with very high specific activities in H2/CO2-grown cells. Based on these findings and on thermodynamic considerations, we propose metabolic schemes that allow, depending on the H2 partial pressure, the chemiosmotic synthesis of 0.14 to 1.5 mol ATP per mol ethanol synthesized from CO2 and H2. IMPORTANCE: Ethanol formation from syngas (H2, CO, and CO2) and from H2 and CO2 that is catalyzed by bacteria is presently a much-discussed process for sustainable production of biofuels. Although the process is already in use, its biochemistry is only incompletely understood. The most pertinent question is how the bacteria conserve energy for growth during ethanol formation from H2 and CO2, considering that acetyl coenzyme A (acetyl-CoA), is an intermediate. Can reduction of the activated acetic acid to ethanol with H2 be coupled with the phosphorylation of ADP? Evidence is presented that this is indeed possible, via both substrate-level phosphorylation and electron transport phosphorylation. In the case of substrate-level phosphorylation, acetyl-CoA reduction to ethanol proceeds via free acetic acid involving acetaldehyde:ferredoxin oxidoreductase (carboxylate reductase).

Authors: J. Mock, Y. Zheng, A. P. Mueller, S. Ly, L. Tran, S. Segovia, S. Nagaraju, M. Kopke, P. Durre, R. K. Thauer

Date Published: 8th Jul 2015

Publication Type: Journal

PubMed ID: 26148714

Citation: J Bacteriol. 2015 Sep;197(18):2965-80. doi: 10.1128/JB.00399-15. Epub 2015 Jul 6.

Created: 6th Dec 2019 at 01:24, Last updated: 8th Dec 2022 at 17:26

Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum

COSMIC

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Clostridium acetobutylicum is able to switch from acidogenic growth to solventogenic growth. We used phosphate-limited continuous cultures that established acidogenic growth at pH 5.8 and solventogenic …

Authors: Christina Grimmler, , , , , , Wolfgang Liebl,

Date Published: 6th Jan 2011

Publication Type: Not specified

PubMed ID: 21212688

Citation:

Created: 23rd Mar 2012 at 16:49, Last updated: 8th Dec 2022 at 17:26

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