Assays

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2 Assays matching the given criteria: (Clear all filters)

Organism:

HepG2 cells (in vitro)

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

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The behaviour of a published MCAD-KO HepG2 cell line was compared to that of a wild-type HepG2 cell line in terms of the changes in acyl-CoA and acylcarnitine levels (measured by HILIC-MS/MS) and gene expression pertaining to CoA metabolism (characterised by qPCR). Additionally, the incorporation of label from stable isotope-labelled pantothenate (vitamine B5) was over 24 hours of exposure to an energetic stressor was also investigated.

Submitter: Christoff Odendaal

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Sequestration of CoA and adaptation of CoA meta...

Organisms: Homo sapiens (batch)

SOPs: No SOPs

Data files: HepG2 CoA labelling data, palmitate/no-glucose ..., HepG2 acyl-CoA measurements, palmitate/low-gluc..., HepG2 acyl-CoA measurements, palmitate/no-gluco..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acylcarnitine measurements, palmitate/low..., HepG2 acylcarnitine measurements, palmitate/no-..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR, palmitate/low-glucose (Fig. S11), HepG2 qPCR, palmitate/no-glucose (Fig. S9)

Snapshots: No snapshots

Created: 9th May 2025 at 14:50, Last updated: 16th May 2025 at 11:45

HepG2 oxygen consumption

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

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Validation of model's ability to predict oxygen consumption flux as measured usign permeabilised cells in an Oroboros Oxygraph. Generates Fig. 2A in the associated publication.

Download "Model_notebooks.rar", unzip, and run: "2, generate-model-Oroboros-validation-[needs(1)]-20221109.nb" and "4, Fig2A-Oroboros-simulation-data-[needs-(1-2-and-3)]-20221109.nb" after running "1, generate-model-20221109.nb"

Submitter: Christoff Odendaal

Biological problem addressed: Validation

Investigation: Mitochondrial fatty acid oxidation in human liver

Study: Model validation

Organisms: Homo sapiens

Models: Model notebooks, odendaal2

SOPs: No SOPs

Data files: HepG2 protein concentration for O2 consumption ..., Oxygen concentration and consumption flux in pe...

Snapshots: No snapshots

Created: 23rd Feb 2023 at 14:41, Last updated: 12th Jun 2023 at 13:30

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