Assays

Absolute quantification of proteins using heavy labeled QconCAT as an internal standard and quantifying the native proteins in the complex sample via scheduled Multiple Reaction Monitoring(MRM) .

Submitter: Praveen kumar Sappa

Assay type: Proteomics

Technology type: Liquid Chromatography Mass Spectrometry

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: B. subtilis_SysMo2_Chemostat_growthrate-salt

Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (chemostat)

SOPs: No SOPs

Data files: 1 hidden item

Snapshots: No snapshots

Created: 21st May 2011 at 15:36, Last updated: 8th Nov 2017 at 14:21

extracellular metabolites

BaCell-SysMO

extracellular metabolite concentrations measured by 1H-NMR

Submitter: Hanna Meyer

Assay type: Metabolomics

Technology type: NMR

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: B. subtilis_SysMo2_Chemostat_growthrate-salt

Organisms: Bacillus subtilis (chemostat)

SOPs: NMR measurment and 1 hidden item

Data files: extracellular metabolites

Snapshots: No snapshots

Created: 27th Jan 2012 at 19:06, Last updated: 8th Nov 2017 at 14:21

intracellular metabolites

BaCell-SysMO

intracellular metabolite measured by GC/MS and LC/MS

Submitter: Hanna Meyer

Assay type: Metabolomics

Technology type: NMR

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: B. subtilis_SysMo2_Chemostat_growthrate-salt

Organisms: Bacillus subtilis (chemostat)

SOPs: 2 hidden items

Data files: intracellular metabolites GC/MS, intracellular metabolites LC/MS

Snapshots: No snapshots

Created: 27th Jan 2012 at 19:15, Last updated: 8th Nov 2017 at 14:21

13C Metabolic Flux Analysis of Bacillus subtilis 168 in continuous high-salinity culture

BaCell-SysMO

Comparison of metabolic flux distribution in carbon core metabolism (EMP, PPP, TCA) of Bacillus subtilis under 3 different conditions: "salt-free" reference, "stress" chemostat, "osmoprotected" chemostat.
Model created using OpenFLUX and Microsoft Excel
Model computed using MatLAB

Submitter: Michael Kohlstedt

Biological problem addressed: Metabolic Network

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: Fluxome analysis of Bacillus subtilis 168 under...

Organisms: No organisms

Models: No Models

SOPs: 1 hidden item

Data files: Flux_analysis_B_subtilis_chemostat_osmoprotection, Flux_analysis_B_subtilis_chemostat_reference, Flux_analysis_B_subtilis_chemostat_stress

Snapshots: No snapshots

Created: 12th Apr 2011 at 11:32, Last updated: 8th Nov 2017 at 14:21

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