Data files

Previously identified gene targets were used as input (see miRNA gene target file).

Literature-curated transcription factor (TF) - miRNA pairs of deregulated miRNAs were extracted from TransmiR (Tong, Cui and Wang 2019 TransmiR). miRNA, target gene, and TF Interaction pairs were visualized in Cytoscape v3.8.2 (Shannon, P. et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research 13, 2498-2504, doi:Doi 10.1101/Gr.1239303 (2003). The missing ...

Previously identified gene targets were used as input (see miRNA gene target file).

Literature-curated transcription factor (TF) - miRNA pairs of deregulated miRNAs were extracted from TransmiR (Tong, Cui and Wang 2019 TransmiR). miRNA, target gene, and TF Interaction pairs were visualized in Cytoscape v3.8.2 (Shannon, P. et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research 13, 2498-2504, doi:Doi 10.1101/Gr.1239303 (2003). The missing ...

Identified gene targets of deregulated miRNAs were used as input. Ontology and pathway GO terms with an adjusted p-value < 0.05 were considered significantly overrepresented

identified gene targets were used as input on the gProfiler website. Ontology and pathway GO terms with an adjusted p-value <0.05 were considered significantly overrepresented.

We obtained PE associated microRNA based on previously mentioned experiments. miRTarBase v8.0 was used to identify gene targets and extract 1000 miRNA - gene pairs with 667 unique genes (Huang et al., 2020). miRTarBase is a database for experimentally validated miRNA-target interactions. To minimize false positives only strong-evidence miRNA-target pairs were considered.

We obtained PE associated microRNA based on previously mentioned experiments. miRTarBase v8.0 was used to identify gene targets and extract 1000 miRNA - gene pairs with 667 unique genes (Huang et al., 2020). miRTarBase is a database for experimentally validated miRNA-target interactions. To minimize false positives only strong-evidence miRNA-target pairs were considered.

The fasta file contains amino acid sequences of genes forming the accessory genome of the Clostridium beijerinckii species. As BLAST might experience some errors with repetitive sequences, here 76 sequences with a single kind of amino acid forming more than 25% of a sequence were discarded.

The fasta file contains amino acid sequences of unique genes found in various Clostridium beijerinckii strains.

The fasta file contains amino acid sequences of genes forming the accessory genome of the Clostridium beijerinckii species.

The fasta file contains amino acid sequences of genes forming the core genome of the Clostridium beijerinckii species.

Quantification of CPM and TCP concentrations in cod liver and bile using gas chromatography

Levels of cortisol+total protein and activities of cholinesterase, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) in plasma of cod exposed to chlorpyrifos-methyl

EROD activity in liver of cod exposed to chlorpyrifos-methyl

Overview of metabolomics results from liver of cod exposed to chlorpyrifos-methyl

Biometrics of IVN fish exposed to chlorpyrifos-methyl

Information connecting RNA seq fastq-files to corresponding fish ID/exposure regime

The four treatment groups were Control (0 mg CPM/kg, with DMSO), 0.5 mg CPM/kg, 4.2 mg CPM/kg and 23.2 mg CPM/kg. Each treatment group consisted of 9 fish. These fish were sampled from 3 different tanks per treatment, with n=9 per treatment. A total of 36 samples were sequenced. For each sample, about 50 million 150 bp paired-end reads were generated.

Master template for hands on session

Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.

ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this apj file, the data for the analysis of the first injection peak is stored. *.apj file

ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this apj file, the data for the analysis of the second injection peak is stored. *.apj file

ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this csv file, the data for the analysis of the both injection peaks is stored. *.csv file

ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in 0.1%Tween-20-KPI buffer (100 mM, pH 7.5) at 25 °C. *.apj file

ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in 0.1%Tween-20-KPI buffer (100 mM, pH 7.5) at 25 °C. *.csv file

Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.

ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.apj file