Molecular characterization and angiogenic properties of extracellular vesicles in healthy and preeclamptic amniotic fluid

The principal research aim of iPLACENTA is to improve our ability to study, model and visualise the placenta. This will contribute to developing diagnostic tests and therapies for pregnancy complications such as preeclampsia and intrauterine growth restriction (IUGR). Placental defects are thought to be the cause of many major pregnancy complications, such as preeclampsia and intra-uterine growth restriction. Preeclampsia affects between 5 and 8 out of every 100 pregnant women and claims the lives ...

Samples underwent size exclusion chromoatography

Full list of detected miRNA which were found to be deregulated in PE-EVs.

Previously identified gene targets were used as input (see miRNA gene target file).

Literature-curated transcription factor (TF) - miRNA pairs of deregulated miRNAs were extracted from TransmiR (Tong, Cui and Wang 2019 TransmiR). miRNA, target gene, and TF Interaction pairs were visualized in Cytoscape v3.8.2 (Shannon, P. et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research 13, 2498-2504, doi:Doi 10.1101/Gr.1239303 (2003). The missing ...

Previously identified gene targets were used as input (see miRNA gene target file).

Literature-curated transcription factor (TF) - miRNA pairs of deregulated miRNAs were extracted from TransmiR (Tong, Cui and Wang 2019 TransmiR). miRNA, target gene, and TF Interaction pairs were visualized in Cytoscape v3.8.2 (Shannon, P. et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research 13, 2498-2504, doi:Doi 10.1101/Gr.1239303 (2003). The missing ...

Identified gene targets of deregulated miRNAs were used as input. Ontology and pathway GO terms with an adjusted p-value < 0.05 were considered significantly overrepresented

identified gene targets were used as input on the gProfiler website. Ontology and pathway GO terms with an adjusted p-value <0.05 were considered significantly overrepresented.

We obtained PE associated microRNA based on previously mentioned experiments. miRTarBase v8.0 was used to identify gene targets and extract 1000 miRNA - gene pairs with 667 unique genes (Huang et al., 2020). miRTarBase is a database for experimentally validated miRNA-target interactions. To minimize false positives only strong-evidence miRNA-target pairs were considered.

We obtained PE associated microRNA based on previously mentioned experiments. miRTarBase v8.0 was used to identify gene targets and extract 1000 miRNA - gene pairs with 667 unique genes (Huang et al., 2020). miRTarBase is a database for experimentally validated miRNA-target interactions. To minimize false positives only strong-evidence miRNA-target pairs were considered.