McWatters A.t. Col-0 WT clock RNA timeseries

Description

Literature data from: 'Defining the robust behaviour of the plant clock gene circuit with absolute RNA timeseries and open infrastructure.' by: Harriet G. McWatters.

    data are col0 8 d seedlings grown on sucrose in either LD 12:12 at 20 oC or WC 27:12 in LL.

Gene expression normalised on btub4 control RNA in same sample at same time. Each datapoint is mean of two biological replicates (each with three technical replicates).

Purpose

Harriet McWatters study to compare clock gene expression with light and temperature entrainment, published in Flis et al. 2015

Comments

Experiment by Harriet McWatters lab in Oxford, perhaps ca. 2007. Published in Flis et al. RS Open Biology 2015. Prep details From Knight, Thomson and McWatters PP 2008: "seedlings were grown on plates containing 1× MS salts with 0.8% to 1% agar and, where indicated, supplemented with 3% (w/v) Suc. In all cases, media pH was corrected to pH 5.8. Unless otherwise stated, seeds were surface-sterilized before being stratified in the dark at 4°C for 48 h prior to transfer to the growth chamber. Plants were grown in Sanyo MLR-350 growth chambers at a constant temperature (20°C). The light level during photoperiods or constant light was 50 μmol m−2 s−1."