SEEK ID: https://fairdomhub.org/assays/2847
Experimental assay
Projects: NExtSEEK Independent Projects
Investigation: BioMicro Center
Study: IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT GENERATION SEQUENCING
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Creators and SubmitterViews: 10
Created: 15th Apr 2026 at 19:19
Last updated: 15th Apr 2026 at 19:34
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Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects
Web page: https://nextseek.mit.edu/
Programme: NExtSEEK
Public web page: Not specified
Organisms: Not specified
Submitter: Charles Demurjian
Studies: IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT GENERATION SEQUENCING
Assays: All Metadata, Genome Alignment - Data Linked, Library Prep - Metadata, Mutational Spectral Analysis - Data Linked, Short Read Sequencing - Data Linked, Variant Calling - Data Linked
Snapshots: No snapshots
Vincent L. Butty, Pranav Patel, Stuart S. Levine

DOI:
Abstract: DNA labelling fluorescent dyes such as ethidium bromide have long been considered to be highly mutagenic during DNA replication. While recent studies have pushed back on this narrative, the intercalative nature of these dyes continues to raise the possibility that these dyes can induce mutations. The iconPCR instrument by n6tec uses fluorescent dyes to measure amplification in real time and to ...
Submitter: Charles Demurjian
Investigation: BioMicro Center
Assays: All Metadata, Genome Alignment - Data Linked, Library Prep - Metadata, Mutational Spectral Analysis - Data Linked, Short Read Sequencing - Data Linked, Variant Calling - Data Linked
Snapshots: No snapshots
Creators: None
Submitter: Charles Demurjian
Investigations: BioMicro Center
Studies: IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT...
Assays: Mutational Spectral Analysis - Data Linked, Variant Calling - Data Linked
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