A.ALN-260403BMC-4

Overview
Related items

UID : A.ALN-260403BMC-4

File_PrimaryData : NA12878_NoDye_Replicate-1_bwa_GRCh38_GIABv3_mapped.bam

Parent : D.SEQ-260403BMC-4

Name : D25-2342

Scientist : Vincent Butty

Protocol : https://fairdomhub.org/sops/899

Genome : GRCh38_GIABv3_no_alt_analysis_set (GIAB masked decoys)

Link_PrimaryData : Not specified

Software : bwa mem v0.7.12-r1039 and VN:0.7.17-r1188; samtools v1.3

Aligner : bwa mem

Creators and Submitter

Creators

Not specified

Submitter

Charles Demurjian

Activity

Views: 6

Created: 15th Apr 2026 at 19:27

Last updated: 15th Apr 2026 at 19:34

Related items

Advanced People list for this Sample with search and filtering

Charles Demurjian

Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects

Institutions: Massachusetts Institute of Technology

Advanced Programmes list for this Sample with search and filtering

NExtSEEK

No description specified

Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects

Web page: https://nextseek.mit.edu/

Advanced Projects list for this Sample with search and filtering

NExtSEEK Independent Projects

No description specified

Programme: NExtSEEK

Public web page: Not specified

Organisms: Not specified

Advanced Investigations list for this Sample with search and filtering

BioMicro Center

NExtSEEK Independent Projects

No description specified

Submitter: Charles Demurjian

Studies: IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT GENERATION SEQUENCING

Assays: All Metadata, Genome Alignment - Data Linked, Library Prep - Metadata, Mutational Spectral Analysis - Data Linked, Short Read Sequencing - Data Linked, Variant Calling - Data Linked

Snapshots: No snapshots

Created: 24th Mar 2026 at 13:43, Last updated: 15th Apr 2026 at 14:16

Advanced Studies list for this Sample with search and filtering

IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT GENERATION SEQUENCING

NExtSEEK Independent Projects

Vincent L. Butty, Pranav Patel, Stuart S. Levine

DOI:

Abstract: DNA labelling fluorescent dyes such as ethidium bromide have long been considered to be highly mutagenic during DNA replication. While recent studies have pushed back on this narrative, the intercalative nature of these dyes continues to raise the possibility that these dyes can induce mutations. The iconPCR instrument by n6tec uses fluorescent dyes to measure amplification in real time and to ...

Submitter: Charles Demurjian

Investigation: BioMicro Center

Assays: All Metadata, Genome Alignment - Data Linked, Library Prep - Metadata, Mutational Spectral Analysis - Data Linked, Short Read Sequencing - Data Linked, Variant Calling - Data Linked

Snapshots: No snapshots

Created: 24th Mar 2026 at 13:45, Last updated: 15th Apr 2026 at 19:30

Advanced Assays list for this Sample with search and filtering