Expertise: translational control of gene expression, gene regulation, protein-protein interactions, protein-RNA interactions, carbon catabolite regulation in Gram positive bacteria, Protein-DNA-interaction, expression systems for pro- and eukayotes, screening systems for nuclear transport and alternative splicing, inducible mammalian cell death.
Wolfgang Hillen Full Professor and Chairman of Microbiology Lehrstuhl für Mikrobiologie Institut für Biologie Friedrich-Alexander Universität Erlangen-Nürnberg Staudtstr. 5 91058 Erlangen, Fed. Rep. of Germany
Date of Birth: April 24, 1948 Place of Birth: Osnabrück, FRG Nationality: German Status: Married Children: Hauke Sven Hillen - May 17, 1987
RESEARCH AND PROFESSIONAL EXPERIENCE
Present Full Professor and Chairman of Microbiology at the Institute of Biology, Friedrich-Alexander ...
Expertise: Microbiology, Molecular Biology, Bacillus subtilis, regulation of gene expression, carbon metabolism, Clostridium, carbon catabolite regulation in Gram positive bacteria, mRNA, overflow metabolism in Bacillus
Tools: Genetic modification, cultivation, northern blot analysis, Molecular biology techniques (RNA/DNA), Optimal experimental design, absolute quantification, qRT-PCR, reporter gene analyses, Western blot analyses, microbiology techniques
Expertise: carbon catabolite regulation in Gram positive bacteria, interaction analyses of transcriptional regulators, analysis and preparation of diagnostic markers for Helicobacter pylori infections Yersiniosis and collagenoses
I studied chemistry in Erlangen and started research on carbon catabolite regulation in Bacillus megaterium in my diploma thesis. During my PhD thesis I was concerned with quantitative analyses of protein-protein and protein-DNA interactions of CcpA, in vivo- and in vitro-characterization of point mutants of CcpA and coeffectors and I contributed to the structural analysis of different CcpA complexes. During a postdoctoral appointment I focused on interaction analyses of PrfA, a regulator of ...
Expertise: Molecular Biology, Bacillus subtilis, functional protein expression, protein-protein interactions, microscopy, Bacterial Cell Biology, carbon catabolite regulation in Gram positive bacteria
Tools: Chromatography, Fluorescence and confocal microscopy, Protein chemical methods (protein overproduction, quantitative western blot analysis, Dynamic modelling, 2-D Gel Electrphoresis, Immunofluorescence, Cell culture, Western blot analyses, interaction analysis techniques especially SPR measurements