Assays

B. subtilis was grown in SMM media with glucose as carbon source and the samples for RNA were harvested OD578nm- 1.0). The stress conditions that were applied over here are growthat 57°C, 16°C, 1.2M Nacl and 37°C(control). All the samples were analysed for transcriptome as biological triplicates.

Submitter: Praveen kumar Sappa

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome of continuously stressed B. subtilis

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)

SOPs: No SOPs

Data files: Continuous growth_16°C_SMM_Growth curve, Continuous growth_57°C_SMM_Growth curve, Contious growth_1.2M NaCl_SMM_Transcritome_Nimb..., Contious growth_16°C_SMM_Transcritome_Nimblegen, Contious growth_57°C_SMM_Transcritome_Nimblegen, Contiuous growth_37°C_SMM(control)_Growth curve., The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 12:45, Last updated: 8th Nov 2017 at 14:21

Tiling Array analysis of shocked B. subtilis cells

BaCell-SysMO

No description specified

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Technology Type

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome of shocked B. subtilis cells

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph) (batch)

SOPs: No SOPs

Data files: The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 13:43, Last updated: 8th Nov 2017 at 14:21

Tiling Array analysis of glucose strarved B. subtilis cells

BaCell-SysMO

B. subtilis was grown in M9 media with glucose as carbon source and the samples were harvested during exponential phase (OD600nm- 0.4), early stationary phase(OD600nm- 1.3), late stationary phase(OD600nm- 1.0). All the samples were analysed for transcriptome as biological triplicates.

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Microarray

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome analysis of glucose starvation in...

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph) (batch)

SOPs: SOP for cultivation of B.Subtilis, Total RNA isolation from B.Subtilis

Data files: M9_shake_flask_exp_trans_stat_transcriptome_nim..., The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 13:47, Last updated: 8th Nov 2017 at 14:21

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