Cultivation and K+ shift of the E. coli strains MG1655 (wildtype) and MG1655 (kdpA4), a (KdpFABC-inactive mutant) Version 1
To induce kdpFABC expression, cells are cultivated in K10 minimal medium (10mM K+) were shifted into K+ limiting growth medium (K0), containing 20 μM K+ by filtration.
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Cultivation and K+-shift.pdf
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Created: 26th Oct 2009 at 14:48
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Version 1 (earliest) Created 26th Oct 2009 at 14:48 by Lisbeth Lyngberg
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Projects: KOSMOBAC
Institutions: University of Aberdeen
I am a research technician at the Institute of Medical Science in Aberdeen, working for Prof. Ian Booth. The topic of our workpackage deals with K+ homostasis in Escherichia coli. I am working with the protein KefF, a regulatory subunit of the potassium channel KefC.
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
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Ion and solute homeostasis in enteric bacteria: an integrated view generated from the interface of modelling and biological experimentation
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