DNA-260402BMC-4
NA12878_lib_EvaGreenPlus
Vincent Butty
WGS
Amplified Sequencing Library
DNA-260402BMC-1
https://fairdomhub.org/sops/900
uL
ng/uL
Library amplified in presence of EvaGreen Plus (1X final concentration) on n6tec iconPCR. Dye concentration and cycle count set above standard iconPCR stop-point parameters to provide a stringent mutagenicity test.
Agilent Fragment Analyzer; qPCR
10.0
150-200
ng/uL
P5/P7 Illumina-compatible adapters
P5/P7 Illumina-compatible adapters
Agilent Fragment Analyzer; qPCR
Creators and Submitter
Views: 5
Created: 15th Apr 2026 at 19:27
Last updated: 15th Apr 2026 at 19:34
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Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects
Web page: https://nextseek.mit.edu/
Programme: NExtSEEK
Public web page: Not specified
Vincent L. Butty, Pranav Patel, Stuart S. Levine
DOI:
Abstract: DNA labelling fluorescent dyes such as ethidium bromide have long been considered to be highly mutagenic during DNA replication. While recent studies have pushed back on this narrative, the intercalative nature of these dyes continues to raise the possibility that these dyes can induce mutations. The iconPCR instrument by n6tec uses fluorescent dyes to measure amplification in real time and to ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: BioMicro Center
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: BioMicro Center
