Glucose Transport in Escherichia coli Mutant Strains with Defects in Sugar Transport Systems
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BiBTeXIn Escherichia coli several systems are known to transport glucose into the cytoplasm. The main glucose uptake system under batch conditions is the glucose phosphoenolpyruvate:carbohydrate phosphotransferase system (glucose-PTS), but also the mannose-PTS, as well as the galactose and maltose transporters can translocate glucose. Mutant strains which lack the EIIBC protein of the glucose-PTS have been previously investigated because their lower rate of acetate formation offers advantages in industrial applications. Nevertheless, a systematic study to analyze the impact of the different glucose uptake systems has not been undertaken. Specifically, how the bacteria cope with the deletion of the major glucose uptake system and which alternative transporters react to compensate for this deficit has not been studied in detail. Therefore, a series of mutant strains were analyzed in aerobic and anaerobic batch cultures, as well as in glucose limited continuous cultivations. Deletion of EIIBC, disturbs glucose transport severely. cAMP-CRP levels rise, induction of the mgl-operon occurs. Nevertheless mgl transcription is not essential, as deletion of this transporter did not affect growth rate; the activities of the remaining transporters seems to be sufficient by induction of the galactose and maltose transporters. Despite the strong up-regulation of mgl under glucose limitations, deletion of this transport-system did not lead to further changes.
SEEK ID: https://fairdomhub.org/publications/185
DOI: 10.1128/JB.01502-12
Projects: SUMO
Publication type: Not specified
Journal: Journal of Bacteriology
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Date Published: 8th Oct 2012
Registered Mode: Not specified
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Created: 19th Dec 2012 at 08:10
Last updated: 8th Dec 2022 at 17:26
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Projects: SUMO
Institutions: Max Planck Institute for Dynamics of Complex Technical Systems
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
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"Systems Understanding of Microbial Oxygen responses" (SUMO) investigates how Escherichia coli senses oxygen, or the associated changes in oxidation/reduction balance, via the Fnr and ArcA proteins, how these systems interact with other regulatory systems, and how the redox response of an E. coli population is generated from the responses of single cells. There are five sub-projects to determine system properties and behaviour and three sub-projects to employ different and complementary modelling ...
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Glucose transporter mutants were analyzed under aerobic and aerobic conditions in batch cultures with glucose as substrate. Acetate formation rates and glucose consumption rates were measured, as well as extracellular cAMP concentrations.
Submitter: Sonja Steinsiek
Assay type: Extracellular Metabolite Concentration
Technology type: Technology Type
Investigation: Analysis of the glucose transport in Escherichi...
Mutant strains which lack one or more of the glucose transport systems were analyzed in aerobic chemostat cultures and compared to batch cultures.
Submitter: Sonja Steinsiek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Analysis of the glucose transport in Escherichi...
MG1655 and mutant strains with defects in glucose transport systems were analyzed in aerobic and anaerobic batch cultures.
Submitter: Sonja Steinsiek
Assay type: Gene Expression Profiling
Technology type: Technology Type
Investigation: Analysis of the glucose transport in Escherichi...
Mutants with defects in glucose transport systems were analyzed in a bioreactor and the transcription of certain uptake systems analyzed. Transcription profiles between batch and chemostat conditions were compared
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
Measurement of by-product formation rates, substrate consumption rates and extracellular concentrations of cAMP under aerobic batch conditions with glucose as substrate.
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
Transcription of glucose uptake transport systems in a mutant background under anaerobic batch conditions.
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
Transcription of glucose uptake transport systems in a mutant background under aerobic batch conditions.
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
Measurement of by-product formation rates, substrate consumption rates and extracellular concentrations of cAMP under anaerobic batch conditions with glucose as substrate.
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
