Glucose Transport in Escherichia coli Mutant Strains with Defects in Sugar Transport Systems


In Escherichia coli several systems are known to transport glucose into the cytoplasm. The main glucose uptake system under batch conditions is the glucose phosphoenolpyruvate:carbohydrate phosphotransferase system (glucose-PTS), but also the mannose-PTS, as well as the galactose and maltose transporters can translocate glucose. Mutant strains which lack the EIIBC protein of the glucose-PTS have been previously investigated because their lower rate of acetate formation offers advantages in industrial applications. Nevertheless, a systematic study to analyze the impact of the different glucose uptake systems has not been undertaken. Specifically, how the bacteria cope with the deletion of the major glucose uptake system and which alternative transporters react to compensate for this deficit has not been studied in detail. Therefore, a series of mutant strains were analyzed in aerobic and anaerobic batch cultures, as well as in glucose limited continuous cultivations. Deletion of EIIBC, disturbs glucose transport severely. cAMP-CRP levels rise, induction of the mgl-operon occurs. Nevertheless mgl transcription is not essential, as deletion of this transporter did not affect growth rate; the activities of the remaining transporters seems to be sufficient by induction of the galactose and maltose transporters. Despite the strong up-regulation of mgl under glucose limitations, deletion of this transport-system did not lead to further changes.


DOI: 10.1128/JB.01502-12

Projects: SUMO

Publication type: Not specified

Journal: Journal of Bacteriology


Date Published: 8th Oct 2012

Registered Mode: Not specified

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Steinsiek, S., & Bettenbrock, K. (2012). Glucose Transport in Escherichia coli Mutant Strains with Defects in Sugar Transport Systems. In Journal of Bacteriology (Vol. 194, Issue 21, pp. 5897–5908). American Society for Microbiology.

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Created: 19th Dec 2012 at 09:10

Last updated: 19th Dec 2012 at 09:11

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