SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae


Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.


PubMed ID: 21651626

Projects: Noisy-Strep

Publication type: Not specified

Journal: Mol. Microbiol.


Date Published: 22nd Jun 2011

Registered Mode: Not specified

Authors: Anita Minnen, Laetitia Attaiech, Maria Thon, Stephan Gruber,

help Creator
Not specified

Views: 3703

Created: 21st Sep 2011 at 10:55

Last updated: 21st Sep 2011 at 10:55

help Attributions


Related items

Powered by
Copyright © 2008 - 2020 The University of Manchester and HITS gGmbH