SOPs

What is a SOP?

17 SOPs visible to you, out of a total of 17

SOP 4B Quenching and extraction of intracellular metabolites

SysMO-LAB

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed L. lactis

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Created: 26th Feb 2010 at 03:25, Last updated: 21st Oct 2016 at 10:06

Quenching and extraction of intracellular metabolites

SysMO-LAB

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Feb 2010 at 03:25, Last updated: 21st Oct 2016 at 10:05

Plasmid transformation lactococci

SysMO-LAB

Method for transformation of plasmids into Lactococcus lactis

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Feb 2010 at 03:25, Last updated: 21st Oct 2016 at 10:04

LAB-Medium

SysMO-LAB

Method for synthesis of LAB-medium sued for the SYSMO-LAB project

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: 1 hidden item

Studies: 1 hidden item

Assays: 1 hidden item

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Created: 26th Feb 2010 at 03:25, Last updated: 21st Oct 2016 at 09:55

Analysis of organic acids in the supernatant

SysMO-LAB

Method for analysis of various organic acids in the medium

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Feb 2010 at 03:25, Last updated: 21st Oct 2016 at 09:55

Measurement of LDH activity

SysMO-LAB

No description specified

Creator: Tomas Fiedler

Submitter: Tomas Fiedler

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Kinetics of L-lactate dehydrogenase from S. pyo...

Assays: Kinetics of L-lactate dehydrogenase from S. pyo...

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Created: 26th Oct 2009 at 14:48, Last updated: 12th May 2016 at 14:25

Measurement of pyruvate kinase activity

SysMO-LAB

No description specified

Creator: Tomas Fiedler

Submitter: Tomas Fiedler

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Oct 2009 at 14:48, Last updated: 12th May 2016 at 14:25

Glucose perturbations in S. pyogenes

SysMO-LAB

Protocol for applying a glucose perturbation in Streptococcus pyogenes.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Oct 2009 at 14:48, Last updated: 11th May 2016 at 15:36

Glucose perturbations

SysMO-LAB

Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Oct 2009 at 14:48, Last updated: 11th May 2016 at 15:32

SOP 7 Glucose-pulse experiments

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch..., The Attic

Studies: Comparative modeling and phosphate dependence f..., Pre-liminary data from Martijn Bekker

Assays: Glucose pulsed L. lactis, Kinetic behavior of intracellular metabolites o..., Kinetic behavior of intracellular metabolites o...

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Created: 26th Feb 2010 at 03:25, Last updated: 11th May 2016 at 15:20

Analysis of organic acid by HPLC

SysMO-LAB

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...

Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...

Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed S. pyogenes

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Created: 26th Oct 2009 at 14:48, Last updated: 11th May 2016 at 15:20

Quenching of lactic acid bacteria

SysMO-LAB

A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

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Created: 26th Oct 2009 at 14:48, Last updated: 11th May 2016 at 15:20

Protocol for glucose perturbations

SysMO-LAB

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

Download

Created: 26th Oct 2009 at 14:48, Last updated: 11th May 2016 at 15:20

SOP 8 Glucose-pulse experiments (adapted for S. pyogenes)

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

Download

Created: 26th Feb 2010 at 03:25, Last updated: 11th May 2016 at 15:20

SOP 5 Analysis of intracellular metabolites

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed L. lactis

Download

Created: 26th Feb 2010 at 03:25, Last updated: 11th May 2016 at 15:20

SOP 6 Analysis of intracellular metabolites

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch..., The Attic

Studies: Comparative modeling and phosphate dependence f..., Pre-liminary data from Martijn Bekker

Assays: Glucose pulsed S. pyogenes, Kinetic behavior of intracellular metabolites o..., Kinetic behavior of intracellular metabolites o...

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Created: 26th Feb 2010 at 03:25, Last updated: 11th May 2016 at 15:20

SOP 2.1 Method for synthesis of LAB-medium used for the SYSMO-LAB project

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...

Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...

Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed L. lactis

Download

Created: 26th Feb 2010 at 03:25, Last updated: 11th May 2016 at 15:20

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