Assays
What is an Assay?Filters
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Single cell clustering is a computational analysis assay that groups individual cells into distinct clusters based on transcriptomic or multi-omic feature similarity, enabling the identification and characterization of cell populations within heterogeneous samples. This data-linked implementation integrates clustering outputs with associated metadata and upstream single-cell sequencing data (such as scRNA-seq), applying algorithms such as k-means, graph-based (e.g., Louvain/Leiden), or hierarchical ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Western blot is a widely used immunodetection technique that separates proteins by molecular weight via gel electrophoresis, transfers them onto a membrane (typically nitrocellulose or PVDF), and detects specific target proteins using primary and secondary antibodies conjugated to reporter molecules such as horseradish peroxidase or fluorescent dyes. Sample preparation typically involves cell or tissue lysis, protein quantification, and denaturation in SDS-containing loading buffer prior to ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay quantifies gene expression at single-cell resolution by linking individual cell transcriptomic profiles to associated experimental or clinical data, enabling integrative analysis across multiple data modalities. Single-cell RNA sequencing (scRNA-seq) technology is employed to capture and measure the transcriptional activity of individual cells, generating high-dimensional expression matrices that are subsequently processed through quality control, normalization, and dimensionality ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Antibody-Dependent Functional Profiling (ADFP) is a multiplexed systems serology approach that quantifies the capacity of antigen-specific antibodies to engage Fc receptors and activate diverse innate immune effector functions. The assay simultaneously measures multiple antibody-dependent activities — including phagocytosis (ADCP, ADNP), complement deposition (ADCD), and NK cell activation (ADNKA) — providing a comprehensive functional fingerprint of the humoral immune response. Antigen-coupled ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay evaluates the ability of bacterial populations to survive under defined restrictive conditions, quantifying survivability metrics through linked experimental data to assess bacterial viability and growth inhibition. The methodology incorporates controlled exposure of bacterial cultures to restrictive agents or environmental stressors, with outcomes measured via standardized enumeration or optical density-based approaches to determine survival rates relative to untreated controls. Results ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
An antibody dependent complement deposition assay measures complement activation following antibody binding. The process detects deposition of complement proteins as an indicator of antibody-mediated immune function. Input: Antibody-treated target cells or particles Output: Complement deposition data file or fluorescence readout.
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with bacterial extraction procedures, documenting the experimental parameters, sample identifiers, reagents, and procedural conditions used during the isolation and extraction of bacterial material from biological or environmental samples. It serves as a standardized record-keeping component that accompanies downstream analytical assays, ensuring traceability and reproducibility by cataloging key variables such as sample source, extraction method, instrument ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Library preparation converts extracted nucleic acids into sequencing-ready libraries. The process includes fragmentation, adapter ligation, and amplification to generate consistent input for sequencing instruments. Input: TIS, CEL, DNA or RNA sample Output: Indexed sequencing library suitable for sequencing assays.
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Digitally barcoded Mtb matrix analysis processes barcode sequencing data from Mycobacterium tuberculosis infection experiments. It quantifies relative barcode abundance to track bacterial population dynamics. Input: Barcode sequencing files Output: Processed abundance matrix showing strain or barcode distribution.
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay quantifies gene expression at single-cell resolution by linking individual cell transcriptomic profiles to associated experimental or clinical data, enabling integrative analysis across multiple data modalities. Single-cell RNA sequencing (scRNA-seq) technology is employed to capture and measure the transcriptional activity of individual cells, generating high-dimensional expression matrices that are subsequently processed through quality control, normalization, and dimensionality ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB