Assays

What is an Assay?

181 Assays visible to you, out of a total of 181

Library Creation - Metadata

Hi-IMPAcTB

DNA sequencing libraries are constructed from genomic DNA extracted from tissue samples through a series of enzymatic and physical processing steps, including DNA fragmentation, end-repair, adapter ligation, and amplification. This workflow prepares nucleic acid material for downstream high-throughput sequencing by converting extracted DNA into indexed, platform-compatible libraries. The resulting libraries enable comprehensive genomic or transcriptomic interrogation of tissue-derived specimens, ...

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: BCG vaccination induces antibacterial effector ...

Organisms: No organisms

SOPs: P.SES-221109-V1_CG000330_Chromium_Next_GEM_Sing..., P.SES-260107-V1_scRNAseq-MM.docx

Data files: No Data files

Snapshots: No snapshots

Created: 4th Mar 2026 at 15:30, Last updated: 27th May 2026 at 20:37

Flow Cytometry - Data Linked

Hi-IMPAcTB

Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: BCG vaccination induces antibacterial effector ...

Organisms: No organisms

SOPs: P.SES-260107-V1_mass-flow-cyto-MM.docx

Data files: No Data files

Snapshots: No snapshots

Created: 4th Mar 2026 at 15:30, Last updated: 27th May 2026 at 20:37

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: BCG vaccination induces antibacterial effector ...

Organisms: No organisms

SOPs: No SOPs

Data files: MM All Metadata.xlsx

Snapshots: No snapshots

Created: 4th Mar 2026 at 20:46, Last updated: 4th Mar 2026 at 20:57

FCS Analysis - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: BCG vaccination induces antibacterial effector ...

Organisms: No organisms

SOPs: P.SES-260107-V1_Quantification&Analysis-MM.docx

Data files: No Data files

Snapshots: No snapshots

Created: 4th Mar 2026 at 15:30, Last updated: 4th Mar 2026 at 20:55

Mass Cytometry - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: BCG vaccination induces antibacterial effector ...

Organisms: No organisms

SOPs: P.SES-260107-V1_mass-flow-cyto-MM.docx

Data files: No Data files

Snapshots: No snapshots

Created: 3rd Mar 2026 at 20:50, Last updated: 4th Mar 2026 at 20:55

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: IFN-γ- GranzymeB + Natural killer cells are ind...

Organisms: No organisms

SOPs: No SOPs

Data files: SM All Metadata.xlsx

Snapshots: No snapshots

Created: 28th Mar 2025 at 17:54, Last updated: 4th Nov 2025 at 15:26

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Protection against reinfection with Mycobacteri...

Organisms: No organisms

SOPs: No SOPs

Data files: G2G All Metadata.xlsx

Snapshots: No snapshots

Created: 20th Oct 2025 at 18:20, Last updated: 22nd Oct 2025 at 14:47

PET-CT Scan - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Protection against reinfection with Mycobacteri...

Organisms: No organisms

SOPs: P.FLY-230523-V1_PET_CT_Imaging

Data files: No Data files

Snapshots: No snapshots

Created: 20th Oct 2025 at 14:52, Last updated: 20th Oct 2025 at 18:44

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: No SOPs

Data files: All Metadata - PG.xlsx

Snapshots: No snapshots

Created: 23rd Apr 2025 at 18:31, Last updated: 23rd Apr 2025 at 19:23

ADCD Analysis - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: P.ALT-241204-V1_Protocol_lmx_comp_deposition_as...

Data files: samples-publish_PG_A.ADCD.xlsx, samples-publish_PG_D.ADCD.xlsx

Snapshots: No snapshots

Created: 22nd Apr 2025 at 18:21, Last updated: 23rd Apr 2025 at 18:58

FC-Receptor Binding Assay - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: P.ALT-241202-V1_Protocol_lmx_FcgR_assay.docx

Data files: samples-publish_PG_AB.xlsx, samples-publish_PG_ABP.xlsx, samples-publish_PG_D.FCRB.xlsx

Snapshots: No snapshots

Created: 22nd Apr 2025 at 18:19, Last updated: 23rd Apr 2025 at 18:49

ELISA Assay - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: P.ALT-241202-V1_LAM_ELISA.docx

Data files: samples-publish_PG_AB.xlsx, samples-publish_PG_ABP.xlsx, samples-publish_PG_D.ELSA.xlsx

Snapshots: No snapshots

Created: 22nd Apr 2025 at 18:17, Last updated: 23rd Apr 2025 at 18:46

Antibody Dependent Functional Profiling - Data Linked

Hi-IMPAcTB

Antibody Dependent Functional Profiling* and Analysis Represents:

ADMP: Macrophage Phagocytosis

ADNP: Neutrophil Phagocytosis

ADDCP: Dendritic Cell Phagocytosis

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: P.ALT-241105-V1_Protocol_phagocytic_assays_BM_c...

Data files: samples-publish_PG_AB.xlsx, samples-publish_PG_CEL.xlsx, samples-publish_PG_D.ADFP.xlsx

Snapshots: No snapshots

Created: 22nd Apr 2025 at 18:22, Last updated: 23rd Apr 2025 at 18:35

Single Cell Clustering - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Antibody-Fab and -Fc features promote Mycobacte...

Organisms: No organisms

SOPs: P.ALT-250314-V1_Analysis-of-infection-scRNA.docx

Data files: samples-publish_PG_A.SCCL.xlsx, samples-publish_PG_A.SCXP.xlsx

Snapshots: No snapshots

Created: 22nd Apr 2025 at 18:16, Last updated: 23rd Apr 2025 at 18:35

Mass Cytometry - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: IFN-γ- GranzymeB + Natural killer cells are ind...

Organisms: No organisms

SOPs: P.SED-241121-V1_Mass-Cytometry-Protocol.docx

Data files: samples-publish_250327_AB.xlsx, samples-publish_250327_ABP.xlsx, samples-publish_250327_D.CYTOF.xlsx, samples-publish_250327_TIS.xlsx

Snapshots: No snapshots

Created: 28th Mar 2025 at 17:51, Last updated: 28th Mar 2025 at 21:51

Mass Cytometry Analysis - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: IFN-γ- GranzymeB + Natural killer cells are ind...

Organisms: No organisms

SOPs: P.SED-241121-V1_Mass-Cytometry-Protocol.docx

Data files: samples-publish_250327_A.CYTOF.xlsx, samples-publish_250327_D.CYTOF.xlsx

Snapshots: No snapshots

Created: 28th Mar 2025 at 17:52, Last updated: 28th Mar 2025 at 21:51

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systematic deconstruction of myeloid cell signa...

Organisms: No organisms

SOPs: No SOPs

Data files: Systematic Granuloma - All Metadata_v1.xlsx

Snapshots: No snapshots

Created: 28th Mar 2025 at 17:10, Last updated: 28th Mar 2025 at 17:26

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: No SOPs

Data files: All Metadata - Training Data.xlsx, All Metadata - Validation Data.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:37, Last updated: 5th Feb 2025 at 22:49

PCR: Validation Data - Data Attached

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241121-V1_qPCR-Protocol.docx

Data files: 111224_PCR.xlsx, samples-publish_KPRF_D.PCR.xlsx, samples-publish_KPRF_SAS_RNA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:33, Last updated: 5th Feb 2025 at 22:46

Bacterial Survivability Restriction Assay: Validation Data - Data Attached

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241120-V1_BSRA-Protocol.docx

Data files: 111224_BRSA.xlsx, samples-publish_KPRF_SAS_BAC.xlsx, samples-publish_KPRF_SAS_CEL.xlsx, samples-publish_KPRF_SAS_D.BSRA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:34, Last updated: 5th Feb 2025 at 22:44

Gene Expression Analysis: Validation Data - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241121-V1_RNAseq-Analysis-Protocol.docx

Data files: samples-publish_KPRF_SAS_A.GEX.xlsx, samples-publish_KPRF_SAS_D.SEQ.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:36, Last updated: 5th Feb 2025 at 22:25

Short Read Sequencing: Validation Data - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241120-V1_RNAseq-Protocol.docx

Data files: samples-publish_KPRF_SAS_D.SEQ.xlsx, samples-publish_KPRF_SAS_DNA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:36, Last updated: 5th Feb 2025 at 21:48

Library Prep: Validation Data - Metadata

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241120-V1_RNAseq-Protocol.docx

Data files: samples-publish_KPRF_SAS_DNA.xlsx, samples-publish_KPRF_SAS_RNA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:35, Last updated: 5th Feb 2025 at 20:46

RNA Extraction: Validation Data - Metadata

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241120-V1_RNAseq-Protocol.docx

Data files: samples-publish_KPRF_SAS_CEL.xlsx, samples-publish_KPRF_SAS_RNA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:33, Last updated: 5th Feb 2025 at 20:00

Cell Extraction: Validation Data - Metadata

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: P.SAS-241120-V1_Bacterial-culture-protocol.docx, P.SAS-241120-V1_Cell-culture-protocol.docx

Data files: samples-publish_KPRF_SAS_BAC.xlsx, samples-publish_KPRF_SAS_CEL.xlsx, samples-publish_KPRF_SAS_MUS.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:30, Last updated: 5th Feb 2025 at 19:47

Gene Expression Analysis: Training Data - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_KPRF_A.GEX.xlsx, samples-publish_KPRF_D.SEQ.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:30, Last updated: 5th Feb 2025 at 19:29

Short Read Sequencing: Training Data - Data Linked

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_KPRF_D.SEQ.xlsx, samples-publish_KPRF_DNA.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:29, Last updated: 5th Feb 2025 at 19:26

Library Prep: Training Data - Metadata

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_KPRF_DNA.xlsx, samples-publish_KPRF_RNA.xlsx, samples-publish_KPRF_TIS.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 18:28, Last updated: 5th Feb 2025 at 19:23

Tissue Collection: Training Data - Metadata

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Systems-level computational translation of mous...

Organisms: No organisms

SOPs: No SOPs

Data files: samples-publish_KPRF_BAC.xlsx, samples-publish_KPRF_MUS.xlsx, samples-publish_KPRF_PAT.xlsx, samples-publish_KPRF_PAV.xlsx, samples-publish_KPRF_TIS.xlsx

Snapshots: No snapshots

Created: 5th Feb 2025 at 17:09, Last updated: 5th Feb 2025 at 19:06

Digitally Barcoded Mtb Matrix Analysis - Data Attached

Hi-IMPAcTB

No description specified

Submitter: Charles Demurjian

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: IMPAcTB

Study: Intravenous BCG-mediated protection against tub...

Organisms: No organisms

SOPs: No SOPs

Data files: A.DBMM-ASIVBCG.xlsx, samples-publishAS-BCG_A.DBMM, samples-publishAS-BCG_D.SEQ

Snapshots: No snapshots

Created: 23rd Jan 2025 at 20:37, Last updated: 3rd Feb 2025 at 22:38

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