Independent Project

Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...

Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...

The family of Desulfobacteraceae comprises mtabolically versatile, completely oxidizing, sulfate-reducing bacteria that play an important role in the global carbon- and sulfor-cycles, mainly in the marine realm. In this study, six different species of Desulfobacteraceae were studied by comparative proteogenomics, i.e. Desulfobacula toluolica Tol2, Desulfococcus multivorans 1be1, Desulfonema limicola, Desulfonema magnum, Desulfosarcina variabilis 3be13 and Desulfobacterium autotrophicum ...

The soluble and membrane protein-enriched fraction of clonal enrichment culture BzS1 were prepared and analyzex by means of nanoLC separation of generated peptides coupled to ESI-iontrap MS/MS.

The soluble protein fraction was subjected to shotgun proteomics (i..e. in-solution digest) applying nanoLC ESI-Iontrap MS/MS and only detections in at least two replicates per condition and bacterium were considered for subsequent analyses. Correspondingly peptide count data was compiled per condition and strain studied.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.