SOP preparation of protein fractions and analysis by nanoLC ESI-iontrap MS/MS Version 1
SEEK ID: https://fairdomhub.org/sops/928?version=1
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BzS1_Sample processing protocol.pdf
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Created: 13th Jul 2026 at 06:48
Last updated: 13th Jul 2026 at 08:18
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Version 1 (earliest) Created 13th Jul 2026 at 06:48 by Lars Wöhlbrand
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Projects: EbN1 Systems Biology, Heat stress response of the red-tide dinoflagellate Prorocentrum cordatum, The nucleus of Prorocentrum cordatum, Conspicuous chloroplast with LHC‒PSI/II‒megacomplex and diverse PBPs in the marine dinoflagellate Prorocentrum cordatum, Proteogneomics of Desulfobacteraceae members, Shotgun and geLC MS/MS data of clonal enrichment culture BzS1
Institutions: Carl von Ossietzky University of Oldenburg Institute for Chemistry and Biology of the Marine Environment (ICBM), General and Molecular Microbiology, General and Molecular Microbiology, Carl von Ossietzky University of Oldenburg Institute for Chemistry and Biology of the Marine Environment (ICBM)
https://orcid.org/0000-0001-7253-3373
Projects: Proteogneomics of Desulfobacteraceae members, Shotgun and geLC MS/MS data of clonal enrichment culture BzS1
Web page: Not specified
Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...
Programme: Independent Project
Public web page: Not specified
Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...
Submitter: Lars Wöhlbrand
Studies: Shotgun and geLC MS/MS analysis of the solble and membrane protein-enric...
Assays: Membrane protein-enriched fraction of clonal enrichment culture BzS1 dur..., Membrane protein-enriched fraction of clonal enrichment culture BzS1 dur..., Soluble protein fraction of clonal enrichment culture BzS1 during growth..., Soluble protein fraction of clonal enrichment culture BzS1 during growth...
The soluble and membrane protein-enriched fraction of clonal enrichment culture BzS1 were prepared and analyzex by means of nanoLC separation of generated peptides coupled to ESI-iontrap MS/MS.
Submitter: Lars Wöhlbrand
Investigation: Shotgun and geLC MS/MS data of clonal enrichmen...
Assays: Membrane protein-enriched fraction of clonal enrichment culture BzS1 dur..., Membrane protein-enriched fraction of clonal enrichment culture BzS1 dur..., Soluble protein fraction of clonal enrichment culture BzS1 during growth..., Soluble protein fraction of clonal enrichment culture BzS1 during growth...
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
Submitter: Lars Wöhlbrand
Assay type: Protein Identification
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Investigation: Shotgun and geLC MS/MS data of clonal enrichmen...
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
Submitter: Lars Wöhlbrand
Assay type: Protein Identification
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Investigation: Shotgun and geLC MS/MS data of clonal enrichmen...
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
Submitter: Lars Wöhlbrand
Assay type: Protein Identification
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Investigation: Shotgun and geLC MS/MS data of clonal enrichmen...
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
Submitter: Lars Wöhlbrand
Assay type: Protein Identification
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Investigation: Shotgun and geLC MS/MS data of clonal enrichmen...
