Shotgun and geLC MS/MS data of clonal enrichment culture BzS1
DownloadDue to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the protein complements of the soluble as well as membrane protein-enriched fractions of cells of strain BzS1 adapted to anaerobic growth with benzene or toluene, respectively, were studied.
DOI: 10.15490/fairdomhub.1.investigation.823.1
Zenodo URL: None
Created at: 13th Jul 2026 at 08:19
Shotgun and geLC MS/MS data of clonal enrichment culture BzS1
Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the protein complements of the soluble as well as membrane protein-enriched fractions of cells of strain BzS1 adapted to anaerobic growth with benzene or toluene, respectively, were studied.
Shotgun and geLC MS/MS analysis of the solble and membrane protein-enriched fraction of clonal enrichment culture BzS1
The soluble and membrane protein-enriched fraction of clonal enrichment culture BzS1 were prepared and analyzex by means of nanoLC separation of generated peptides coupled to ESI-iontrap MS/MS.
Membrane protein-enriched fraction of clonal enrichment culture BzS1 during growth with benzene
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
SOP preparation of protein fractions and analysis by nanoLC ESI-iontrap MS/MS
No description specified
- BzS1_Sample processing protocol.pdf
Membrane protein-enriched fraction of clonal enrichment culture BzS1 during growth with toluene
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
SOP preparation of protein fractions and analysis by nanoLC ESI-iontrap MS/MS
No description specified
- BzS1_Sample processing protocol.pdf
Soluble protein fraction of clonal enrichment culture BzS1 during growth with benzene
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
SOP preparation of protein fractions and analysis by nanoLC ESI-iontrap MS/MS
No description specified
- BzS1_Sample processing protocol.pdf
Soluble protein fraction of clonal enrichment culture BzS1 during growth with toluene
Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.
SOP preparation of protein fractions and analysis by nanoLC ESI-iontrap MS/MS
No description specified
- BzS1_Sample processing protocol.pdf
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Created: 13th Jul 2026 at 08:19
Last updated: 13th Jul 2026 at 08:21
