Assays

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.

The soluble protein fraction was subjected to shotgun proteomics (i..e. in-solution digest) applying nanoLC ESI-Iontrap MS/MS and only detections in at least two replicates per condition and bacterium were considered for subsequent analyses. Correspondingly peptide count data was compiled per condition and strain studied.