Data files

Master table describing all the samples included in MOA Investigation; studies are descrubed using MIAPPE (Minimum Information About a Plant Phenotyping Experiment).

R code for the hormone analysis.

R code for differential expression analysis of sRNA-Seq.

Some additional files required for the R code (phenodata, feature data i.e. the GAL file and Agi4x44PreProcess downloaded package, as it is not maintained on BioConductor anymore).

Code used for the analysis of microarray data.

Archive containing some phenotyping images (.tar.gz).

phasiRNAs and their counts identified in the MOA samples for sRNA-Seq.

phasiRNAs and their RPM normalised counts identified in the MOA samples for sRNA-Seq.

Differential expression of phasiRNAs between various comparison groups (Sheet: comparisons), with log2-fold changes and Benjamini-Hochberg FDR corrected p-values given.

vsiRNAs and their counts identified in the MOA samples for sRNA-Seq.

vsiRNAs and their RPM normalised counts identified in the MOA samples for sRNA-Seq.

miRNA stem-loop RT-qPCR for sRNA-Seq data validation. sRNA expression results obtained by sRNA-seq were validated by stem-loop RT-qPCR. For validation experiments, the same RNA samples as used for sRNA-Seq were analysed.

RT-qPCR assays for sRNA-Seq validation described using the MIQE standard.