SOPs
What is a SOP?Filters
Creator: Martijn Bekker
Submitter: The JERM Harvester
This method describes how to derivatize the N-glutathionylspermidine and trypanothione produced by T. brucei trypanothione synthetase under in vivo-like conditions
Creator: Alejandro Leroux
Submitter: Alejandro Leroux
Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein
Creator: Femke Mensonides
Submitter: Maksim Zakhartsev
Sample preparation - SOP for sampling, preparation of cell extracts, and general assay set-up
Creator: Femke Mensonides
Submitter: Maksim Zakhartsev
The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes
Creator: Hanan Messiha
Submitter: Maksim Zakhartsev