SOPs
What is a SOP?Filters
Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)
Creator: Maksim Zakhartsev
Submitter: Maksim Zakhartsev
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Creator: Lina Patricia Barreto Parra
Submitter: Lina Patricia Barreto Parra
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Submitter: The JERM Harvester
Purpose:
Creator: Jose Ramos
Submitter: The JERM Harvester
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
Creator: Lisbeth Lyngberg
Submitter: Lisbeth Lyngberg
Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A
Submitter: Jay Moore
Creators: Louise Thomas, Maggie Smith
Submitter: Jay Moore
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins
Creator: Miguel Curto Rubio
Submitter: The JERM Harvester
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins
Creator: Miguel Curto Rubio
Submitter: The JERM Harvester
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
Creators: Jacky Snoep, Theresa Kouril
Submitter: Jacky Snoep
Creators: Louise Thomas, Maggie Smith
Submitter: Jay Moore
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Creator: Martijn Bekker
Submitter: Martijn Bekker
Method of how to perform the purification of glyoxalase II as well as kinetics assays.
Creator: Lisbeth Lyngberg
Submitter: Lisbeth Lyngberg
Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.
Creator: Lisbeth Lyngberg
Submitter: Lisbeth Lyngberg
A method of how to measure methylglyoxal present in the growth medium.
Creator: Lisbeth Lyngberg
Submitter: Lisbeth Lyngberg
