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Lisa Wasserstrom

About Lisa Wasserstrom:
No description specified

SEEK ID: https://fairdomhub.org/people/614

Location:  Sweden

Expertise: Molecular Biology, Metabolic Engineering, Saccharomyces cerevisiae, Genetics

Tools: Genetic modification, Genetics, Metabolic Engineering, Molecular Biology, Fermentation

ORCID: https://orcid.org/0000-0002-5131-876X

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Project positions

XyloCut (Lund University)

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XyloCut

D-xylose is a major component of lignocellulose and is after D-glucose the most abundant monosaccharide on earth. However, D-xylose cannot be naturally utilised by several industrially relevant microorganisms. On the way to a strong bio-based economy in Europe, this widely available feedstock has to be made accessible for the sustainable microbial synthesis of value-added chemical building blocks to be used in a broad range of applications. The project aims at engineering Corynebacterium glutamicum
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Programme: ERASysAPP

Public web page: https://www.erasysapp.eu/calls/2nd-call/xylocut

Lund University

Country:  Sweden

City: Lund

Web page: http://www.lunduniversity.lu.se/

Characterization of the Weimberg Pathway in Caulobacter crescentus
XyloCut

Abstract (Expand)

Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α-ketoglutarate in five steps without carbon loss. This … is an interesting pathway for heterologous expression in other organisms in order to enable xylose utilization in biorefinery processes. C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h−1, 0.05 h−1, and 0.15 h−1 respectively. Growth was found to be significantly inhibited at sugar concentration of 20 g L−1, shown primarily by an increased lag phase. Enzyme activity assays showed that the Weimberg pathway was active in cells grown, not only on xylose but also on arabinose. No activity was found for growth on glucose. Furthermore, substantial amounts of α-ketoglutarate—up to a yield of 0.4 g g−1—was excreted during growth on xylose, but no other extracellular intermediates in the Weimberg pathway were detected during growth on xylose. Apparently, C. crescentus is not well adapted for efficient growth on high xylose levels, and responds by an extended lag phase and secretion of α-ketoglutarate.

Authors: Henrik Almqvist, Sara Jonsdottir Glaser, Celina Tufvegren, Lisa Wasserstrom, Gunnar Lidén

Date Published: 1st Jun 2018

Publication Type: Not specified

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