Assays
What is an Assay?Filters
Clock genes under Far Red light - 3
| Property | Value |
|---|---|
| BioDare ID | 12723746109930 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Purpose
Analysis of the main clock markers under strict FR ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
080115H1
| Property | Value |
|---|---|
| BioDare ID | 2629 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
080115H2
| Property | Value |
|---|---|
| BioDare ID | 2630 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
210814H2
| Property | Value |
|---|---|
| BioDare ID | 1926 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
Partner experiment: 210814H1
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
210814H1
| Property | Value |
|---|---|
| BioDare ID | 1925 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
190614H1
| Property | Value |
|---|---|
| BioDare ID | 1642 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
190614H2
| Property | Value |
|---|---|
| BioDare ID | 1643 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
270313H1: 21L 7Dx4 LLx3 (T-28)
| Property | Value |
|---|---|
| BioDare ID | 13649842953637 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 21L 7D Experimental conditions: 21L7Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime during the first three imaging days appear to be absent, ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200313H2: 7L 21Dx4 LLx3 (T-28)
| Property | Value |
|---|---|
| BioDare ID | 13643892291642 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
290313H2: 15L 5Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13649886952409 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime during the first three imaging days appear to be absent, using the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
270313H3: 12L-12Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13650039169340 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')
Purpose
'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200313H3: 12L-12Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13644021710933 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')
Purpose
'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
060213H3: 12B-12Dx4 BBx3
| Property | Value |
|---|---|
| BioDare ID | 13615365050841 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)
Purpose
Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Imaging script started at ZT+7mins, therefore ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
030413H3: 12B-12Dx4 BB3
| Property | Value |
|---|---|
| BioDare ID | 13661271159675 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)
Purpose
Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
AT0048 4x 18:6-DD
| Property | Value |
|---|---|
| BioDare ID | 12730752301671 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
4 days of 18hrs light, 6hrs dark into 3 days constant dark
Purpose
changes to photoperiod
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
BW-detached_LL-LL_#14
| Property | Value |
|---|---|
| BioDare ID | 13394382079593 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 14 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-detached_LL-LL_#16
| Property | Value |
|---|---|
| BioDare ID | 13394383188744 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 16 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-detached_LD-LL_#06
| Property | Value |
|---|---|
| BioDare ID | 13394381111502 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 06 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-detached_LD-LL_#03
| Property | Value |
|---|---|
| BioDare ID | 13394379875121 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 03 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LL-LL_#56
| Property | Value |
|---|---|
| BioDare ID | 13394446929060 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 56 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LL-LL_#46
| Property | Value |
|---|---|
| BioDare ID | 13394446784669 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 46 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LL-LD_#31
| Property | Value |
|---|---|
| BioDare ID | 13394443003897 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown under constant light and imaged under LD. Plant number 31 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LL-LL_#37
| Property | Value |
|---|---|
| BioDare ID | 13394444364628 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 37 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LL-LL_#58
| Property | Value |
|---|---|
| BioDare ID | 13394446996851 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 58 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
BW-intact_LD-LL_#23
| Property | Value |
|---|---|
| BioDare ID | 13394434885385 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Intact leaves of CCA1:LUC grown under LD and imaged under constant light. Sample number 23 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
030810H3 SD-LD
| Property | Value |
|---|---|
| BioDare ID | 13982027442235 |
| Author | Sarah Hodge |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 8L:16D (120umol m-2s-1) for 7 days. Experimental Conditions: 2 days 8L:16D, 4 days 16L:8D (red and blue light 60umolm-2s-1)
Purpose
Re-entrainment dynamics during transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Dixon Laura
Study: Dixon et al. (2014)
BW-detached_LL-LL_#20
| Property | Value |
|---|---|
| BioDare ID | 13394437504756 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 20 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
Edwards et al. (2010); WT in different light conditions.
| Property | Value |
|---|---|
| BioDare ID | 13227622661196 |
| Author | Edwards, K.D. |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'Quantitative analysis of regulatory flexibility under changing environmental conditions.' by: Edwards, K.D..
Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kieron D Edwards
Study: Edwards et al. (2010)
Flis et al. TiMet clock gene comparison across photoperiods
| Property | Value |
|---|---|
| BioDare ID | 3967 |
| Author | Anna Flis |
| Institution | Max Planck Inst. Golm |
| License | CC_BY |
Description
Literature data from: 'Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis' by: Anna Flis.
Col plants were grown in soil under 6, 8, 12 and 18h photoperiod for 21 days, and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2016)
Clock gene RNAs under RD-RR in clock mutants, expt 4
| Property | Value |
|---|---|
| BioDare ID | 13228357183121 |
| Author | Locke |
| Institution | |
| License |
Description
Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.
First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 4 of 4.
Purpose
Testing effects of clock ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
