060313H1: 5L15Dx4 LLx3 (T-20)

Description

Plants entrained in 5L 15D Experimental conditions: 5L15Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

130313H2: 12F-12Dx4 FFx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

060313H2: 12F-12Dx4 FFx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Far-Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

200313H3: 12L-12Dx4 LLx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

270313H3: 12L-12Dx4 LLx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

030413H3: 12B-12Dx4 BB3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

060213H3: 12B-12Dx4 BBx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

Imaging script started at ZT+7mins, therefore ...

200213H3: 12B-12Dx4 BB3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

130213H3: 12R-12Dx4 RRx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

270213H3: 12R-12Dx4 RRx3

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Red)

Purpose

Test how the phytochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

AT0080: 4x18:6-DD

Description

LUC imaging 18L:6D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

Pedro prepared by editing groups from previous template

AT0048 4x 18:6-DD

Description

4 days of 18hrs light, 6hrs dark into 3 days constant dark

Purpose

changes to photoperiod

Comments

AT0043: 4x 12:12-DD

Description

LUC imaging 12:12 blue+red into constant darkness

Purpose

testing acute light responses at dawn and dusk

Comments

AT0078: 4x6:18-DD

Description

LUC imaging 6L:18D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

BW-detached_LL-LL_#14

Description

Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 14 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-detached_LD-LL_#06

Description

Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 06 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

BW-detached_LL-LL_#16

Description

Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 16 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-detached_LD-LL_#03

Description

Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 03 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

BW-intact_LL-LL_#46

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 46 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-intact_LL-LL_#56

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 56 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-intact_LL-LL_#37

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 37 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-intact_LL-LD_#31

Description

Intact leaves of CCA1:LUC grown under constant light and imaged under LD. Plant number 31 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

BW-intact_LD-LL_#23

Description

Intact leaves of CCA1:LUC grown under LD and imaged under constant light. Sample number 23 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

...

BW-intact_LL-LL_#58

Description

Intact leaves of CCA1:LUC grown and imaged under constant light. Sample number 58 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

BW-detached_LL-LL_#20

Description

Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 20 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.

Purpose

Testing ...

030810H3 SD-LD

Description

Plants entrained in 8L:16D (120umol m-2s-1) for 7 days. Experimental Conditions: 2 days 8L:16D, 4 days 16L:8D (red and blue light 60umolm-2s-1)

Purpose

Re-entrainment dynamics during transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and ...

Edwards et al. (2010); WT in different light conditions.

Description

Literature data from: 'Quantitative analysis of regulatory flexibility under changing environmental conditions.' by: Edwards, K.D..

Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases ...

Effects of GA on clock in WT and prr9prr7

Description

Purpose

To test whether GA has any effect on circadian clock period in the WT, and whether this effect is altered in the prr9prr7 mutant.

Comments

Flis et al. TiMet clock gene comparison across photoperiods

Description

Literature data from: 'Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis' by: Anna Flis.

Col plants were grown in soil under 6, 8, 12 and 18h photoperiod for 21 days, and ...

Q-PCR LD (12:12) 17C LHY

Description

Literature data from: 'Temp' by: Aurora Pinas-Fernandez.

Wild-type and clock-mutant seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees, every 2h, for 24h.

Purpose

...