Assays
What is an Assay?Protein copy number at 6h, 12h, 24h, 48h, 72h, 96h, average values and SD for the measurements
Submitter: Niels Zondervan
Assay type: Proteomics
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Proteomics analysis
Metabolomics time series measurements for internal metabolites for 6h, 24h and 48h for multiple experiments. Largely based on MAss spectrometry, bioluminescence kits to measure NAD, NADH at 24h, other time points are infered from relative measurements times the absolute measurements at 24h.
Submitter: Niels Zondervan
Assay type: Experimental Assay Type
Technology type: Mass Spectrometry
Investigation: Modelling of M. pneumoniae metabolism
Study: Metabolomics measurements
Measurements of external metabolites based on growth curve data. Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate
Submitter: Niels Zondervan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Metabolomics measurements
Simple overview of all samples used for training, internal validation by copasi en external validation. Overview of samples metadata, mean metabolite concentration and enzyme concentrations used in the model. Only metabolites present in the model are shown.
Submitter: Niels Zondervan
Assay type: Experimental Assay Type
Technology type: Mass Spectrometry
Investigation: Modelling of M. pneumoniae metabolism
Study: Metabolomics measurements
Comparison of Kcat values from the model and values from literature.
Submitter: Niels Zondervan
Assay type: Enzymatic Assay
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Core Model training
Submitter: Niels Zondervan
Assay type: Transcriptional Profiling
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism