output/Activity assays Trans2.docx

Version 1

National Institute of Biology, Department of Biotechnology and Systems Biology projects

SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.

Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036

Short Name: T31_mealybug Title: Mealybug genome and transcriptome analyses Description: Searching for enzymes involved in the mealybug pheromone biosynthesis pathways. These pheromones are known to be released by virgin females. Phenodata: ./phenodata_20190523.txt Featuredata: Principal investigator: Heribert Warzecha, Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes

Short Name: P3_expressionEcoli Title: Expression of IDS candidates in E. coli Description: IDS candidate sequences are expressed in E. coli and tested for enzyme activities Raw Data: Principal investigator: Heribert Warzecha License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes

Short Name: Trans2-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans2 Description: Expression in E. coli and activity testing of Trans2 pISA Assay creation date: 2020-11-04 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pCR-BluntTrans02_2.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-11-05 Sample purification ...