_S_P3_expressionEcoli
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Short Name: P3_expressionEcoli Title: Expression of IDS candidates in E. coli Description: IDS candidate sequences are expressed in E. coli and tested for enzyme activities Raw Data: Principal investigator: Heribert Warzecha License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
SEEK ID: https://fairdomhub.org/studies/1036
Projects: SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Contr...
Study position: 1
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Created: 16th May 2022 at 10:26
Last updated: 6th Nov 2024 at 15:25
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T31_mealybug Title: Mealybug genome and transcriptome analyses Description: Searching for enzymes involved in the mealybug pheromone biosynthesis pathways. These pheromones are known to be released by virgin females. Phenodata: ./phenodata_20190523.txt Featuredata: Principal investigator: Heribert Warzecha, Špela Baebler License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Studies: Investigation files, _S_P3_expressionEcoli, _S_P4_AdPathGenes, _S_P4_Pcitri_IsoSeq, _S_P4_Pcitri_genome, _S_P4_Pcitri_genome_extSamples, _S_P4_Pcitri_tr1, _S_P4_Pcitri_tr1tr2combo, _S_P4_Pcitri_tr2, _S_P4_cand_all, _S_P4_cand_genomic
Assays: _A_01-CoEXpNetViz_tr2-coexpr, _A_01_NGI-IsoSeq, _A_01_RNAseq-CLC, _A_01_assembly2-rnaSPAdes, _A_01_mapToGenomeAll-STAR, _A_01_renameFASTA-Bioinfo, _A_02-CoEXpNetViz_gen-coexpr, _A_02_CDHIT-Bioinfo, _A_02_assembly2-IPS, _A_02_cDNAcupcake-dry, _A_02_limma-R, _A_02_mapToGenomeAll_limma-R, _A_03-IPS, _A_03_CDHIT2-Bioinfo, _A_03_limma_min50counts-R, _A_03_mapAs2ToGenome-STAR, _A_04_limma_min10counts-R, _A_04_mapIllumina-STAR, _A_04_mapReadsToAs2-STAR, _A_05_As2_tr_limmaDE-R, _A_05_DE_limma-R, _A_05_assembly1-rnaSPAdes, _A_06_assembly1-IPS, _A_07_mapAs1ToGenome-STAR, _A_08_mapReadsToAs1-STAR, _A_09_As1_tr_limmaDE-R, _A_C98F-EcoliExpr, _A_IDIsearch-Bioinfo, _A_IPS-bioinfo, _A_MAST-bioinfo, _A_MEME-bioinfo, _A_MSA-bioinfo, _A_MeUppS-EcoliExpr, _A_Node14610-EcoliExpr, _A_Node14610DmTP-EcoliExpr, _A_OtherMealybugCandidates-Alignment, _A_Pc1599-EcoliExpr, _A_Pc23180-EcoliExpr, _A_Pc2704-EcoliExpr, _A_Pc32607-EcoliExpr, _A_Pc7366II-EcoliExpr, _A_PcCis24-EcoliExpr, _A_Strep_CLDS-bioinfo, _A_TPS_phy-bioinfo, _A_Terzyme-bioinfo, _A_Trans1153-EcoliExpr, _A_Trans2-EcoliExpr, _A_Trans39-EcoliExpr, _A_Trans57-EcoliExpr, _A_Trans64-EcoliExpr, _A_Trans94-EcoliExpr, _A_candidatemining-bioinfo, _A_g14825_recon-bioinfo, _A_smallIDSsubunit-motifs, _I_T31_mealybug-files, _S_P3_expressionEcoli-files, _S_P4_AdPathGenes-files, _S_P4_Pcitri_IsoSeq-files, _S_P4_Pcitri_genome-files, _S_P4_Pcitri_genome_extSamples-files, _S_P4_Pcitri_tr1-files, _S_P4_Pcitri_tr1tr2combo-files, _S_P4_Pcitri_tr2-files, _S_P4_cand_all-files, _S_P4_cand_genomic-files
Files connected to study: _S_P3_expressionEcoli
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: C98F-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: C98F chimeric protein Description: Expression in E. coli and activity testing of C98F chimeric protein pISA Assay creation date: 2019-05-07 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/C98F.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-05-08 Sample purification protocol (file ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: MeUppS-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from Moranella endobia Description: Expression in E. coli and activity testing pISA Assay creation date: 2020-07-01 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XMeUppS.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30 Date of sample collection: 2020-07-02 Sample purification protocol ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Node14610-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: A candidate sequence from P. citri Node14610 Description: Expression in E. coli and activity testing of Node14610 pISA Assay creation date: 2019-05-14 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XNode14610.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-05-15 Sample ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Node14610DmTP-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Node14610 without a predicted mitochondria targeting peptide Description: Expression in E. coli and activity testing pISA Assay creation date: 2019-05-20 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XNode14610DmTP.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Pc1599-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc1599 Description: Expression in E. coli and activity testing of Pc1599 pISA Assay creation date: 2019-07-01 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc1599.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-07-02 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Pc23180-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc23180 Description: Expression in E. coli and activity testing of Pc23180 pISA Assay creation date: 2019-09-03 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc23180.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-09-04 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Pc2704-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc2704 Description: Expression in E. coli and activity testing of Pc2704 pISA Assay creation date: 2019-06-05 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc2704.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-06-06 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Pc32607-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc32607 Description: Expression in E. coli and activity testing of Pc32607 pISA Assay creation date: 2019-05-30 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc32607.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-05-31 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Pc7366II-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc7366II Description: Expression in E. coli and activity testing of Pc7366II pISA Assay creation date: 2020-01-29 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5x_Pc7366II_sequenced..gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-01-30 ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: PcCis24-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Description: Expression in E. coli and activity testing of PcCis24 pISA Assay creation date: 2020-07-08 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5Xcis24.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 18 Date of sample collection: 2020-07-09 Sample purification protocol ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans1153-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans1153 Description: Expression in E. coli and activity testing of Trans1153 pISA Assay creation date: 2019-08-13 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5Xtrans1153.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-08-14 Sample ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans2-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans2 Description: Expression in E. coli and activity testing of Trans2 pISA Assay creation date: 2020-11-04 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pCR-BluntTrans02_2.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-11-05 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans39-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans39 Description: Expression in E. coli and activity testing of Trans39 pISA Assay creation date: 2020-11-08 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pCR-BluntTrans39_3.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-11-09 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans57-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans57 Description: Expression in E. coli and activity testing of PcTrans57 pISA Assay creation date: 2020-11-25 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5Xtrans57.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-11-26 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans64-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans64 Description: Expression in E. coli and activity testing of Trans64 pISA Assay creation date: 2020-11-04 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pCR-Blunttrans64.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-11-05 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
Short Name: Trans94-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Trans94 Description: Expression in E. coli and activity testing of Trans94 pISA Assay creation date: 2020-08-04 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5Xtrans94.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2020-08-05 Sample purification ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T31_mealybug
Study: _S_P3_expressionEcoli
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans39-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans57-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans64-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans94-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc7366II-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_PcCis24-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans2-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Node14610DmTP-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc1599-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc23180-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc2704-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc32607-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_C98F-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_MeUppS-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Node14610-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans64-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans94-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans57-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans64-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans94-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans2-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans39-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans57-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans2-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans39-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc2704-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc32607-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc7366II-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_PcCis24-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Trans1153-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T31_mealybug/_S_P3_expressionEcoli/_A_Pc2704-EcoliExpr/
Creator: Marko Petek
Submitter: Marko Petek
