Data files

This file contains the R-script to analyse single nuclei data previously processed with kallisto and bustools. The analyses utilizes the Seurat, harmony and RNAvelocity package.

This file contains the detailed UNIX commands for mm10 index file creation (suitable for RNAvelocity) for kallisto as well as the commands used for the alignment with kallisto and the subsequent quantification with bustools.

Overview of the quality control for the RNAseq short read data after quality filtering of the reads

Overview for quality control of mapping statistics, after mapping processed reads to reference genomes

Repository for code used in data analysis (mainly for RNAseq) and for generating summary tables and overviews.

Files for the reference genomes utilized in the analysis are summarised in this repository.

Overivew of RNAseq and proteomics samples with respective accessions to access the raw data on ENA or PRIDE respectively.

Pride project for raw and search data for AXX-Cn samples: LNU-AXX-Si00-CnB-P-B6ST-Pr_180min LNU-AXX-Si00-CnB-P-B7ST-Pr_180min LNU-AXX-Si00-CnB-P-B8ST-Pr_180min

Raw proteomics data for L. ferriphilum planktonic samples

Raw proteomics data for S. thermosulfidooxidans samples

Raw data for tertiary mixture, planktonic cells, proteomics

Raw data for , Ac + St or Lf + St planktonic cells from leaching experiments

Raw proteomics data for St + Ac planktonic cells

Raw data for Ac + St leaching experiments proteomics data, with Ac 10x inoculum sizing

Raw data for Ac+St planktonic cells, leaching experiments with St 10x incoulum sizing

Parameters used in maxQuant analysis

Overview of the quality control for the RNAseq short read data before quality filtering of the reads

Raw data for mixed culture biofilm proteomics

Samples: UDE-SAL8-7O-M-B-Pr-2 UDE-SAL8-7O-M-B-Pr UDE-SAL7-Pc20-7M-P-B-Pr_2uL UDE-SAL7-A7-M-B-Pr UDE-SAL7-7C-M-B-Pr UDE-SAL7-7A-M-B-Pr

Microscopy images of A.Caldus (A), L.ferriphilum (L), S.thermosulfidooxidans (S) biofilms on chalcopyrite particles. (individual,e.g. SXX or mixed, e.g. SAL, cultures)

Timepoints: Day1, Day3, Day7, Day14, Day21

Raw data for mixed cultures of S. thermosulfidooxidans and L.ferriphilum

Raw data for L.ferriphilum biofilm proteomics data

This file contains the IDs, adj. p-values and official gene names of the top 100 marker genes (where applicable) for each of the identified cluster.

Log2 fold changes after drug treatment/serum stimulation of MAPK and AKT pathway nodes

Log2 fold changes after drug treatment/serum stimulation of MAPK and AKT pathway nodes

Log2 fold changes after drug treatment/serum stimulation of MAPK and AKT pathway nodes

Data files for the conversion of XYL to KG, via the sequential addition of the Caulobacter crescentus Weimberg pathway enzymes, XDH, XLA, XAD, KDXD, KGSADH.

Initial rate kinetics for the α-ketoglutarate semialdehyde dehydrogenase of Caulobacter crescentus, α-ketoglutarate semialdehyde and NAD saturation, and α-ketoglutarate, NADH and 2-keto-3-deoxy-D-xylonate inhibition.

Initial rate kinetics for the 2-keto-3-deoxy-D-xylonate dehydrates of Caulobacter crescentus, 2-keto-3-deoxy-D-xylonate saturation and inhibitor titrations.

Initial rate kinetics for the xylonate dehydratase of Caulobacter crescentus, xylonate saturation.

Initial rate kinetics for the xylonolactonase reaction of Caulobacter crescentus, xylonolactone saturation for the enzyme catalysed reaction, and for the non-enzymatic reaction.