Data files

Salmon feed switch experiment: Lipidomics data (POS mode) of liver samples.

Muscle samples (fresh water sampling) - Identification of compounds based on the LipidBlast database.

Negative mode lipidomics of muscle samples from saltwater sampling.

Muscle samples (salt water sampling) - Identification of compounds based on the LipidBlast database.

Liver samples (salt water sampling) - Identification of compounds based on the LipidBlast database.

Liver samples (fresh water sampling) - Identification of compounds based on the LipidBlast database.

Liver samples (fresh water sampling) - Identification of compounds based on the LipidBlast database.

Muscle samples (fresh water sampling) - Identification of compounds based on the LipidBlast database.

Salmon feed experiment: Lipidomic data (NEG mode) of muscle samples from fresh water sampling.

Salmon feed experiment: Lipidomic data (NEG mode) of liver samples from fresh water sampling.

Negative mode lipidomics of liver samples from saltwater sampling.

Positive mode metabolomics of muscle samples from saltwater sampling.

Muscle samples (salt water sampling) - Identification of compounds based on the LipidBlast database.

Columns: Compound 8.60_759.5784n Compound ID GPCho(12:0/22:1) Accepted? Adducts M+H, M+Na Formula C42H82NO8P Score 51,1 Fragmentation Score 62,1 Mass Error (ppm) 0,808672852 Isotope Similarity 94,59106017 Theoretical Isotope Distribution 100 - 47 - 12.5 - 2.39 - 0.367 Link http://nonlinear.com/redirect/outbound?p=lipidblast&param=GPCho%2812%3A0%2F22%3A1%29 Description GPCho(12:0/22:1) Neutral ...

Liver samples (salt water sampling) - Identification of compounds based on the LipidBlast database.

Columns: Compound 8.60_759.5784n Compound ID GPCho(12:0/22:1) Accepted? Adducts M+H, M+Na Formula C42H82NO8P Score 51,1 Fragmentation Score 62,1 Mass Error (ppm) 0,808672852 Isotope Similarity 94,59106017 Theoretical Isotope Distribution 100 - 47 - 12.5 - 2.39 - 0.367 Link http://nonlinear.com/redirect/outbound?p=lipidblast¶m=GPCho%2812%3A0%2F22%3A1%29 Description GPCho(12:0/22:1) Neutral mass (Da) ...

Source code is at https://gitlab.com/digisal/GSF1_metabolomics/. The report loads smoothly in Chrome, but is very slow to load in Firefox.

Salmon feed experiment: Lipidomic data (POS mode) of muscle samples from fresh water sampling.

Salmon feed experiment: Lipidomic data (POS mode) of liver samples from fresh water sampling.

Positive mode metabolomics of liver samples from saltwater sampling.

Positive mode metabolomics of the same muscle tissue sample at multiple levels of dilution.

Samples of 40 45 50 55 60 mg were diluted in the same volume, then analysed. The resulting data describe the same tissue at different levels of dilution. From this we can devise some numerical post-processing that would make all samples "similar" based on the mass of tissue. This post-processing could then be applied to later samples given their mass.

NOTE: Sample does not come from the salmon feed-switch ...

Excel file summarizing:

• Name of the RNAseq study
• Orion path were the .fastq files are stored
• Year the libraries were sequenced
• short description

This is the fatty acid profile data from the freshwater portion of the feed switch trial. The last column has the sample ID eg: D0_MA-L-1/9_6 (Day 0, MA- Marine oil, L-liver, 1/9 sept 1, fish number 6

Fatty acid Systematic Name C14:0 Myristic acid myristin-syre C16:0 Palmitic acid palmitin-syre C16:1n7 Palmitoleic palmitolein-syre C17:0 C17:1 C18:0 stearic acid stearin-syre 18:1n9c oleic acid olje-syre C18:2n6c linoleic acid linolsyre C20:1 eicosenoic acid C18:3n3 linolenic acid linolensyre C20:2 ...

Fatty acid Systematic Name

C14:0 Myristic acid myristin-syre C16:0 Palmitic acid palmitin-syre C16:1n7 Palmitoleic palmitolein-syre C17:0 C17:1 C18:0 stearic acid stearin-syre 18:1n9c oleic acid olje-syre C18:2n6c linoleic acid linolsyre C20:1 eicosenoic acid C18:3n3 linolenic acid linolensyre C20:2 eicosadienoic C22:1 cetoleic acid cetolein-syre C20:3n3 eicosatrienoic acid C20:4n6 arachidonic acid arakidon-syre ARA C22:2 C20:5n3 eicosapentaenoic acid EPA C24:1 nervonic acid C22:5n3 docosapentaenoic ...

This is the fatty acid profile data from the freshwater portion of the feed switch trial. eg: D0_MA_M_6 (Day 0, MA- Marine oil, L-liver, fish number 6)

Fatty acid Systematic Name C14:0 Myristic acid myristin-syre C16:0 Palmitic acid palmitin-syre C16:1n7 Palmitoleic palmitolein-syre C17:0 C17:1 C18:0 stearic acid stearin-syre 18:1n9c oleic acid olje-syre C18:2n6c linoleic acid linolsyre C20:1 eicosenoic acid C18:3n3 linolenic acid linolensyre C20:2 eicosadienoic C22:1 cetoleic acid cetolein-syre ...

This is the fatty acid profile data from the seawater portion of the feed switch trial. eg: D0_MA_M_6 (Day 0, MA- Marine oil, Muscle, fish number 6)

Fatty acid Systematic Name C14:0 Myristic acid myristin-syre C16:0 Palmitic acid palmitin-syre C16:1n7 Palmitoleic palmitolein-syre C17:0 C17:1 C18:0 stearic acid stearin-syre 18:1n9c oleic acid olje-syre C18:2n6c linoleic acid linolsyre C20:1 eicosenoic acid C18:3n3 linolenic acid linolensyre C20:2 eicosadienoic C22:1 cetoleic acid cetolein-syre ...

Fatty acid methyl ester (FAME) analysis showing which fatty acids were in the samples, though not what specific lipids they were part of (triacylglycerides, phospholipids, etc.) https://en.wikipedia.org/wiki/Fatty_acid_methyl_ester

Values in Ark1!A2:AO21 are probably mass percent of total FAME, including C19:0 added for calibration. Sample identifiers are lab-local; GSF1 identifiers are in Ark5!A4:U22 but suffer from typos. Use caution and see below.

This workbook is a mess:

• Results appear in ...

Results of gas chromatography of fatty acid composition of feeds used in Study "GSF1: Salmon feed-switch experiment vegetable and fish oil 2015-2016", https://fairdomhub.org/studies/144.

Feed samples are identified by their EWOS feed number. Fatty acids are identified by ChEBI ID. Numbers in table are mass percentage of lipid fraction.

Details on recipes, pellet size etc for each feed ID are in https://fairdomhub.org/data_files/1308.

(The above clarifications required some detective work, which ...

At the GenoSysFat startup meeting 2015-04-22, Dominic Nanton of EWOS described the feeds they made for GenoSysFat.

Describes recipes with linseed oil (LNO) and palm oil (PO) to provide the 18:3n-3 precursor to EPA and DHA and match saturation of 100% fish oil (FO) control diet at ca 0.7 mass% EPA+DHA.

Page 2: Overview Page 3-4: Description of feeds. Feed no., Code, Initial fish weight (g), Feed size (mm), Fish number (3000), Feed (kg) Page 5-7: Feed formulations. Feed number, Feed name, Feed size ...

A file which combine previous metabolomics samples ID to new sample ID file (https://fairdomhub.org/data_files/1330)

Information for salmon genes, including transcript length used for calculating transcrip per million (TPM) value

Read counts for each sample