Assays
What is an Assay?Filters
The behaviour of a published MCAD-KO HepG2 cell line was compared to that of a wild-type HepG2 cell line in terms of the changes in acyl-CoA and acylcarnitine levels (measured by HILIC-MS/MS) and gene expression pertaining to CoA metabolism (characterised by qPCR). Additionally, the incorporation of label from stable isotope-labelled pantothenate (vitamine B5) was over 24 hours of exposure to an energetic stressor was also investigated.
Submitter: Christoff Odendaal
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Mitochondrial fatty acid oxidation in human liver