FLOWERING LOCUS C Controls Temperature Response of the Arabidopsis Clock

Description

Literature data from: 'FLOWERING LOCUS C mediates natural variation in the high-temperature response of the Arabidopsis circadian clock' by: Edwards KD.

    Authors have analyzed FLC alleles in near-isogenic lines and induced mutants to eliminate other candidate genes. They showed

that FLC lengthened the circadian period specifically at 278C, contributing to temperature compensation of the circadian clock. Known upstream regulators of FLC expression in flowering time pathways similarly controlled its circadian effect. They sought to identify downstreamtargets of FLCregulation in the molecular mechanismof the circadian clock using genome-wide analysis to identify FLC-responsive genes and 3503 transcripts controlled by the circadian clock. A Bayesian clustering method based on Fourier coefficients allowed us to discriminate putative regulatory genes.Among rhythmic FLC-responsive genes, transcripts of the transcription factor LUX ARRHYTHMO (LUX) correlated in peak abundance with the circadian period in flc mutants. Mathematical modeling indicated that the modest change in peak LUX RNA abundance was sufficient to cause the period change due to FLC, providing a molecular target for the crosstalk between flowering time pathways and circadian regulation.

Purpose

Identify downstream targets of FLCregulation in themolecular mechanism of the circadian clock using genome-wide analysis

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