BW-detached_LD-LL_#03
| Property | Value |
|---|---|
| BioDare ID | 13394379875121 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown in LD and imaged under constant light. Sample number 03 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing spatiotemporal pattern of rhythmic gene expression to assay intercellular coupling, and comparing the strength of intercellular coupling in LL with entrainment in LD.
Comments
Start date is the date when imaging started.
Numerical data are provided as a 2D x time matrix in Matlab format (.mat), and as a text file with coordinates above each column of data. Please see the attached ReadMe file for notes on the data.
SEEK ID: https://fairdomhub.org/assays/2931
Experimental assay
Projects: BioDare1
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: Arabidopsis thaliana
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Creators and SubmitterViews: 78
Created: 18th Jun 2026 at 13:15
Last updated: 23rd Jul 2026 at 13:12
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Projects: Millar group, BioDare1
Institutions: University of Edinburgh, School of Biological Sciences, University of Edinburgh
https://orcid.org/0000-0002-1352-7245
Expertise: Data Management, Python, R, Programming, microscopy, fluorescence protein fusions, Software Engineering
Tools: Data Management, Python, R, Fluorescence and confocal microscopy, Time-lapse fluorescence microscopy Flow cytometry, Fluorecence based reporter gene analyses/single cell analyses
Research Software Engineer in the Biological Research Data Management team (BioRDM) at the University of Edinburgh.
The Centre for Engineering Biology is the University of Edinburgh's research organisation in interdisciplinary, Synthetic and Systems Biology, founded in 2023 as the successor to SynthSys, itself founded in 2012 as the successor to the Centre for Systems Biology at Edinburgh (CSBE). CSBE was newly founded in 2007. The Centre for Engineering Biology is a hub organisation that links across multiple, major funding awards, including an Engineering Biology Mission Hub, and EPSRC C-loop.
Projects: Millar group, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, TiMet, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository, BioDare1
Web page: https://biology.ed.ac.uk/centre-engineering-biology
Experimental data that were previously hosted on the original version of BioDare (biodare.ed.ac.uk), which opened in 2009, please see https://doi.org/10.1007/978-1-4939-0700-7_2. BioDare was designed as a repository of rhythmic biological data, especially for 24-hour circadian rhythms, with specialised analytical routines for period, phase and amplitude estimation on each data timeseries. Most rhythms tested were measured using gene expression, either directly as RNA levels, or indirectly using ...
Programme: Centre for Engineering Biology (prev. SynthSys)
Public web page: https://biodare2.ed.ac.uk
Start date: 7th Jul 2006
End date: 21st Dec 2017
Organisms: Arabidopsis thaliana, Neurospora crassa
Collection of experiments created by Wenden Benedicte on the original BioDare and automatically transferred to FAIRDOM Hub.
Submitter: Daniel Thedie
Studies: Unpublished, Wenden et al. (2012)
Assays: BW-detached_LD-LL_#03, BW-detached_LD-LL_#06, BW-detached_LL-LL_#14, BW-detached_LL-LL_#16, BW-detached_LL-LL_#20, BW-intact_LD-LL_#23, BW-intact_LL-LD_#31, BW-intact_LL-LL_#37, BW-intact_LL-LL_#46, BW-intact_LL-LL_#56, BW-intact_LL-LL_#58, CCA1 and CCR2 markers in elf4 mutant under Far Red light, Clock genes under Far Red light - 3, Clock genes under Far Red light - 5, Clock genes under Far Red light - 6, Wenden_qPCR_clock_cFR, WT and elf4, Wenden_qPCR_clock_cFR, WT and phyA
Snapshots: No snapshots
Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf
Wenden et al. (2012)
DOI: 10.1073/pnas.1118814109
Submitter: Daniel Thedie
Investigation: Wenden Benedicte
Assays: BW-detached_LD-LL_#03, BW-detached_LD-LL_#06, BW-detached_LL-LL_#14, BW-detached_LL-LL_#16, BW-detached_LL-LL_#20, BW-intact_LD-LL_#23, BW-intact_LL-LD_#31, BW-intact_LL-LL_#37, BW-intact_LL-LL_#46, BW-intact_LL-LL_#56, BW-intact_LL-LL_#58
Snapshots: No snapshots
CCA1LDgrownLLimaged_7 (from BioDare)
Toner PNAS 12 all (from BioDare)
README (from BioDare)
Plant03 (from BioDare)
List of samples used in the assay (extracted from original BioDare metadata and converted to csv)
Original BioDare metadata, converted to json format
Readme file
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